PMID- 3982226
OWN - NLM
STAT- MEDLINE
DCOM- 19850520
LR  - 20190701
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 36
IP  - 16
DP  - 1985 Apr 22
TI  - Increased cytosolic calcium in cystic fibrosis neutrophils effect on 
      stimulus-secretion coupling.
PG  - 1561-7
AB  - A disorder of calcium homeostasis has been related to the pathogenesis of Cystic 
      Fibrosis (CF). The Authors have studied the relationship between the cytosolic 
      free calcium concentration ([Ca2+]i), the amount of Ca2+ released from endogenous 
      stores and the secretory response in CF neutrophils. Significantly elevated 
      resting [Ca2+]i and depressed Ca2+ release induced by the chemotactic peptide 
      N-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP) is present in CF 
      neutrophils. In the absence of exogenous Ca2+ the secretory response of CF 
      neutrophils after a weak stimulus such as Cytochalasin B (CB) is greater than in 
      normal neutrophils, while a depressed secretion of azurophilic granules is 
      evident in CF neutrophils stimulated by CB + FMLP. The data confirm the 
      hypothesis of an altered Ca2+ homeostasis in CF cells. Cystic Fibrosis (CF), an 
      autosomal recessive exocrinopathy, is characterized by secretory abnormalities 
      and ion transport dysfunctions (for review see 1,2). Since intracellular Ca2+ 
      seems to play a role in stimulus-secretion coupling and ion movements, several 
      aspects of Ca2+ homeostasis have been investigated in CF. The total Ca2+ content 
      has been reported to be increased in fibroblast cultures and in lymphocytes 
      (3,4,5) and mitochondrial Ca2+ uptake was found elevated in fibroblast cultures 
      (6). An elevated free cytosolic calcium concentration ([Ca2+]i) has been recently 
      reported in buccal epithelial cells (7), while normal concentration has been 
      found in lymphocytes and Epstein Barr virus transformed lymphoblasts (5,8). The 
      present paper shows the results of a study in human neutrophils, a cell whose 
      several functions such as secretion, movement and respiratory burst are in some 
      way regulated by Ca2+. The data report that in neutrophils of CF patients the 
      resting [Ca2+]i is higher and the secretory response is partly modified.
FAU - Cabrini, G
AU  - Cabrini G
FAU - De Togni, P
AU  - De Togni P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Aminoquinolines)
RN  - 3CHI920QS7 (Cytochalasin B)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - O448IDK23O (Quin2)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Aminoquinolines/metabolism
MH  - Calcium/*blood
MH  - Cystic Fibrosis/*blood
MH  - Cytochalasin B/pharmacology
MH  - Cytosol/metabolism
MH  - Humans
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*metabolism
EDAT- 1985/04/22 00:00
MHDA- 1985/04/22 00:01
CRDT- 1985/04/22 00:00
PHST- 1985/04/22 00:00 [pubmed]
PHST- 1985/04/22 00:01 [medline]
PHST- 1985/04/22 00:00 [entrez]
AID - 0024-3205(85)90380-7 [pii]
AID - 10.1016/0024-3205(85)90380-7 [doi]
PST - ppublish
SO  - Life Sci. 1985 Apr 22;36(16):1561-7. doi: 10.1016/0024-3205(85)90380-7.