PMID- 3987930
OWN - NLM
STAT- MEDLINE
DCOM- 19850531
LR  - 20170920
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 43
IP  - 4
DP  - 1985 Apr
TI  - Protein O-carboxylmethyltransferase in spermatozoa from normal and infertile men.
PG  - 636-45
AB  - In confirmation of several reports, suspensions of normal washed human 
      spermatozoa exposed to a nonionic detergent exhibited considerable activity of 
      the enzyme protein O-carboxylmethyltransferase (PCM), which catalyzes the methyl 
      esterification of carboxyl groups of dicarboxylic amino acid residues in 
      proteins. Various methods for assay of human spermatozoal PCM levels were 
      evaluated, and some properties of the enzyme were studied. Normal human 
      spermatozoa appear to be devoid of other types of protein methyltransferases that 
      catalyze N-methylations of arginyl or lysyl residues in proteins. Spermatozoal 
      PCM levels in infertile patients with motile sperm cells tended to be somewhat 
      higher than those of normal control subjects, especially in those instances where 
      the spermatozoal populations contained abnormally high proportions of immature 
      forms of spermatozoa. Although totally immotile spermatozoa obtained from certain 
      patients exhibited very low PCM activities (as recently reported by other 
      investigators), in this study no invariant relationship between zero motility 
      indexes and spermatozoal PCM was observed. These results are discussed in light 
      of various current hypotheses regarding the functions of PCM in animal cells.
FAU - Hatch, R
AU  - Hatch R
FAU - Harvey, S E
AU  - Harvey SE
FAU - Williams-Ashman, H G
AU  - Williams-Ashman HG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - EC 2.1.1.- (Protein Methyltransferases)
RN  - EC 2.1.1.- (Protein O-Methyltransferase)
SB  - IM
MH  - Cell Separation
MH  - Centrifugation
MH  - Humans
MH  - Infertility, Male/*enzymology
MH  - Male
MH  - Protein Methyltransferases/*metabolism
MH  - Protein O-Methyltransferase/*metabolism
MH  - Sperm Count
MH  - Sperm Maturation
MH  - Sperm Motility
MH  - Spermatozoa/*enzymology/physiology
EDAT- 1985/04/01 00:00
MHDA- 1985/04/01 00:01
CRDT- 1985/04/01 00:00
PHST- 1985/04/01 00:00 [pubmed]
PHST- 1985/04/01 00:01 [medline]
PHST- 1985/04/01 00:00 [entrez]
AID - S0015-0282(16)48510-9 [pii]
PST - ppublish
SO  - Fertil Steril. 1985 Apr;43(4):636-45.