PMID- 4004801
OWN - NLM
STAT- MEDLINE
DCOM- 19850725
LR  - 20190501
IS  - 0264-6021 (Print)
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Linking)
VI  - 227
IP  - 3
DP  - 1985 May 1
TI  - The glutathione S-transferases in selenium and vitamin E deficiency.
PG  - 823-31
AB  - Preliminary experiments confirmed the work of others showing that the total 
      glutathione peroxidase (GSH-px) activity of rat liver supernatant fraction may be 
      resolved into two peaks of activity (peaks I and II) by gel filtration, and that 
      peak I is the selenium-containing enzyme and peak II is another peroxidase 
      indistinguishable from glutathione S-transferase (GST). In selenium and vitamin E 
      deficiency, the total activity of the GSH-px became very low, and the total 
      activity of GST with 1-chloro-2,4-dinitrobenzene (CDNB) as substrate was 
      enhanced. Study of the time course of these changes as deficiency progressed 
      indicated that the stimulus for the rise in GST (CDNB) activity was the fall in 
      GSH-px activity which preceded it. The peroxidase activity of GST was found to 
      reside only in the GST AA, B and B2 forms of the enzyme, which were shown to be 
      respectively a homodimer of the Yc subunit, a homodimer of the Ya subunit and a 
      heterodimer of the YaYc subunit. As vitamin E and selenium deficiency progressed, 
      the B2 and AA forms of the enzyme showed enhanced activity, which was interpreted 
      as implying that the Yc subunit of the enzyme becomes enriched as a consequence 
      of the withdrawal of selenium from the animal's diet. Densitometric measurements 
      of the Yc and Ya subunits confirmed that the amount of the Yc subunit was nearly 
      doubled in selenium deficiency, relative to the Ya subunit.
FAU - Mehlert, A
AU  - Mehlert A
FAU - Diplock, A T
AU  - Diplock AT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - H6241UJ22B (Selenium)
SB  - IM
MH  - Animals
MH  - Chromatography
MH  - Chromatography, Gel
MH  - Densitometry
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Glutathione Peroxidase/metabolism
MH  - Glutathione Transferase/*metabolism
MH  - Liver/*enzymology
MH  - Male
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Selenium/*deficiency
MH  - Vitamin E Deficiency/*enzymology
PMC - PMC1144911
EDAT- 1985/05/01 00:00
MHDA- 1985/05/01 00:01
PMCR- 1985/11/01
CRDT- 1985/05/01 00:00
PHST- 1985/05/01 00:00 [pubmed]
PHST- 1985/05/01 00:01 [medline]
PHST- 1985/05/01 00:00 [entrez]
PHST- 1985/11/01 00:00 [pmc-release]
AID - 10.1042/bj2270823 [doi]
PST - ppublish
SO  - Biochem J. 1985 May 1;227(3):823-31. doi: 10.1042/bj2270823.