PMID- 4006904
OWN - NLM
STAT- MEDLINE
DCOM- 19850806
LR  - 20220511
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 4
IP  - 3
DP  - 1985 Mar
TI  - Integration of transfected LTR sequences into the c-raf proto-oncogene: 
      activation by promoter insertion.
PG  - 693-8
AB  - A malignant cell line (clone S1) isolated after co-transfection of normal NIH3T3 
      DNA and Moloney leukemia virus long terminal repeat (Mo-LTR) sequences has 
      previously been described to contain an activated c-raf oncogene. Here, we report 
      the isolation by molecular cloning and the structural analysis of the 
      LTR-activated c-raf gene. As shown by Southern blot and nucleotide sequence 
      analyses, the transfected Mo-LTR sequences integrated into the 5th intron of the 
      endogenous c-raf proto-oncogene. This intragenic LTR insertion led to the 
      expression of high levels of LTR-U5-c-raf hybrid transcripts indicating an 
      initiation of transcription from the Mo-LTR promoter. Transcriptional activation 
      of c-raf is accompanied by the synthesis of large amounts of cytoplasmic c-raf 
      protein. Immunoblot analysis suggests that the proteins encoded by the 
      LTR-activated c-raf gene are truncated compared with the normal c-raf gene 
      product(s). Our results indicate a promoter insertion mechanism of c-raf 
      activation.
FAU - Molders, H
AU  - Molders H
FAU - Defesche, J
AU  - Defesche J
FAU - Muller, D
AU  - Muller D
FAU - Bonner, T I
AU  - Bonner TI
FAU - Rapp, U R
AU  - Rapp UR
FAU - Muller, R
AU  - Muller R
LA  - eng
PT  - Journal Article
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - *Cell Transformation, Neoplastic
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - DNA, Viral/genetics
MH  - Gene Amplification
MH  - Gene Expression Regulation
MH  - Genetic Linkage
MH  - Mice
MH  - Moloney murine leukemia virus/*genetics
MH  - *Oncogenes
MH  - Promoter Regions, Genetic
MH  - Repetitive Sequences, Nucleic Acid
MH  - Transcription, Genetic
PMC - PMC554244
EDAT- 1985/03/01 00:00
MHDA- 1985/03/01 00:01
PMCR- 1986/03/01
CRDT- 1985/03/01 00:00
PHST- 1985/03/01 00:00 [pubmed]
PHST- 1985/03/01 00:01 [medline]
PHST- 1985/03/01 00:00 [entrez]
PHST- 1986/03/01 00:00 [pmc-release]
AID - 10.1002/j.1460-2075.1985.tb03685.x [doi]
PST - ppublish
SO  - EMBO J. 1985 Mar;4(3):693-8. doi: 10.1002/j.1460-2075.1985.tb03685.x.