PMID- 4008141
OWN - NLM
STAT- MEDLINE
DCOM- 19850814
LR  - 20190908
IS  - 0192-0561 (Print)
IS  - 0192-0561 (Linking)
VI  - 7
IP  - 2
DP  - 1985
TI  - Inhibition of human and guinea pig complement by heparin fractions differing in 
      affinity for antithrombin III or in average molecular weight.
PG  - 271-80
AB  - Heparin comprises a mixture of structurally related molecules. Affinity for 
      antithrombin III (AT III) is a prerequisite for its anticoagulant activity, which 
      also is dependent on its molecular weight. In this study heparin fractions 
      prepared by affinity chromatography on immobilized AT III and by gel filtration 
      chromatography were compared for their ability to inhibit complement mediated 
      haemolysis and both classical and alternative pathway C3 activation as measured 
      by crossed immunoelectrophoresis. In normal human serum, inhibition of haemolysis 
      and of heat-aggregated IgG (HAGG) as well as zymosan induced C3 activation by 
      heparin was found to be independent of its AT III affinity and of its molecular 
      weight (range 4800-17,000), on a weight basis. In guinea pig serum similar 
      results were obtained for inhibition of HAGG induced C3 activation, but 
      inhibition of haemolysis showed a marked molecular weight dependency and was also 
      reduced for the fraction with low affinity for AT III. This may reflect a species 
      difference in the haemolytic action of human and guinea pig complement. It is 
      concluded that inhibition of human complement by heparin is independent of its 
      anticoagulant activity and of its size and it is suggested that a fraction of 
      heparin with reduced risk for bleeding and platelet aggregation is a potential 
      anti-inflammatory agent.
FAU - Ekre, H P
AU  - Ekre HP
LA  - eng
PT  - Journal Article
PL  - England
TA  - Int J Immunopharmacol
JT  - International journal of immunopharmacology
JID - 7904799
RN  - 0 (Complement C3)
RN  - 9000-94-6 (Antithrombin III)
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Animals
MH  - Antithrombin III/metabolism
MH  - Complement C3/*antagonists & inhibitors
MH  - Complement Pathway, Alternative
MH  - Complement Pathway, Classical
MH  - Guinea Pigs
MH  - Hemolysis/drug effects
MH  - Heparin/*analysis/metabolism/pharmacology
MH  - Humans
MH  - Molecular Weight
MH  - Protein Binding
EDAT- 1985/01/01 00:00
MHDA- 1985/01/01 00:01
CRDT- 1985/01/01 00:00
PHST- 1985/01/01 00:00 [pubmed]
PHST- 1985/01/01 00:01 [medline]
PHST- 1985/01/01 00:00 [entrez]
AID - 0192-0561(85)90036-0 [pii]
AID - 10.1016/0192-0561(85)90036-0 [doi]
PST - ppublish
SO  - Int J Immunopharmacol. 1985;7(2):271-80. doi: 10.1016/0192-0561(85)90036-0.