PMID- 4008276
OWN - NLM
STAT- MEDLINE
DCOM- 19850820
LR  - 20190829
IS  - 0301-5564 (Print)
IS  - 0301-5564 (Linking)
VI  - 82
IP  - 4
DP  - 1985
TI  - Microfluorometric estimates of proteins associated with murine hepatocyte and 
      thymocyte nuclei, residual structures, and nuclear matrix derivatives.
PG  - 331-9
AB  - Isolated diploid hepatocyte and thymocyte nuclei and their derivatives ("residual 
      structures" and nuclear matrices, as defined by Kaufmann et al. 1981) were 
      evaluated by microfluorometry following reaction with the following 
      fluorochromes: brilliant sulfaflavine (BSF) used at pH 2.8 for the demonstration 
      of total protein; acridine orange (AO) used at pH 9.0 to reveal acidic groups of 
      proteins; and 3-(4-maleimidylphenyl)-7-diethylamino-4-methylcoumarin (CPM) used 
      under conditions required to demonstrate the sum of sulfhydryl (SH) and disulfide 
      (SS) groups of proteins. The results suggested that the proteins reacting with AO 
      and CPM differed from each other and from those revealed by fluorochroming with 
      BSF. In every comparison, hepatocyte nuclei and their derivatives were more 
      fluorescent than the respective populations of thymocyte nuclei and their 
      derivatives. In material fluorochromed with BSF and AO, nuclear matrices were 
      less fluorescent than residual structures, which, in turn, were less fluorescent 
      than intact nuclei. In contrast, nuclear matrices fluorochromed with CPM were 
      less fluorescent than intact nuclei but more fluorescent (paradoxically) than 
      residual structures. The ratios of the total fluorescence values of hepatocyte 
      and thymocyte nuclei fluorochromed with BSF changed significantly during 
      extractions required to produce residual structures and nuclear matrices, while 
      comparable ratios in material fluorochromed with AO or CPM did not change 
      significantly. Comparisons of the ratios of the fluorescence values of intact 
      nuclei and their derivatives in a variety of combinations yielded complex and 
      variable results.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Curtis, S K
AU  - Curtis SK
FAU - Cowden, R R
AU  - Cowden RR
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Germany
TA  - Histochemistry
JT  - Histochemistry
JID - 0411300
RN  - 0 (Coumarins)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Isoquinolines)
RN  - 0 (Nucleoproteins)
RN  - 2391-30-2 (brilliant sulfaflavine)
RN  - 76877-33-3 (N-(4-(7-diethylamino-4-methylcoumarin-3-yl)phenyl)maleimide)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Cell Fractionation/methods
MH  - Cell Nucleus/*analysis
MH  - Coumarins
MH  - Female
MH  - Flow Cytometry/*methods
MH  - Fluorescent Dyes
MH  - Isoquinolines
MH  - Liver/analysis/*cytology
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Nucleoproteins/*analysis
MH  - Thymus Gland/analysis/*cytology
EDAT- 1985/01/01 00:00
MHDA- 1985/01/01 00:01
CRDT- 1985/01/01 00:00
PHST- 1985/01/01 00:00 [pubmed]
PHST- 1985/01/01 00:01 [medline]
PHST- 1985/01/01 00:00 [entrez]
AID - 10.1007/BF00494061 [doi]
PST - ppublish
SO  - Histochemistry. 1985;82(4):331-9. doi: 10.1007/BF00494061.