PMID- 4020134
OWN - NLM
STAT- MEDLINE
DCOM- 19850919
LR  - 20141120
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 135
IP  - 3
DP  - 1985 Sep
TI  - Immune response to nucleic acid antigens and native DNA by human peripheral blood 
      lymphocytes in vitro.
PG  - 1772-7
AB  - The immunogenicity of DNA fragments (either oligonucleotide (oligo) or total DNA 
      digest) covalently linked to keyhole limpet hemocyanin (oligo-KLH or DNA-KLH) was 
      tested with peripheral blood lymphocytes (PBL) from 63 systemic lupus 
      erythematosus patients (SLE) in vitro. PBL from 10 normal individuals and 11 
      rheumatoid arthritis (RA) patients served as controls. Antibodies to three 
      nucleic acid antigens (oligo, denatured DNA (d-DNA), and native DNA (n-DNA] were 
      assayed in supernatants of cultured lymphoid cells by a sensitive solid-phase 
      radioimmunoassay. More than 50% of SLE and RA patient lymphoid cells formed 
      spontaneous antibodies to one or several nucleic acid antigens. In contrast, only 
      two normals did. After in vitro challenge with oligo-KLH or DNA-KLH, cultured 
      lymphocytes of more than 50% of SLE patients formed antibodies to one or several 
      nucleic acid antigens. Similar results were obtained in PBL from RA patients. In 
      SLE patients, the response to both antigens was either monospecific or 
      polyspecific, but DNA-KLH appeared to raise a greater proportion of antibody to 
      n-DNA than oligo-KLH. A greater proportion of patients with active disease 
      responded in vitro compared with those with inactive disease. A mixture of oligo 
      together with KLH was not immunogenic in vitro. Oligo-KLH or DNA-KLH did not 
      raise antibody to an irrelevant antigen, ovalbumin. Of particular interest, PBL 
      from seven of 10 normal subjects formed antibody to n-DNA after challenge in 
      vitro with oligo-KLH. The data support the view that DNA fragments could be an 
      important immunogen in SLE. Furthermore, this study provides an in vitro model to 
      test the tolerogenicity of similar fragments of DNA linked to self carrier 
      molecules such as gamma-globulin.
FAU - Bastian, D
AU  - Bastian D
FAU - Borel, H
AU  - Borel H
FAU - Sasaki, T
AU  - Sasaki T
FAU - Steinberg, A D
AU  - Steinberg AD
FAU - Borel, Y
AU  - Borel Y
LA  - eng
GR  - AI 21163/AI/NIAID NIH HHS/United States
GR  - AM 33465/AM/NIADDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Nucleic Acids)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Antibody Formation
MH  - Antibody Specificity
MH  - Arthritis, Rheumatoid/*immunology
MH  - Cells, Cultured
MH  - DNA/*immunology
MH  - Humans
MH  - In Vitro Techniques
MH  - Lupus Erythematosus, Systemic/*immunology
MH  - Lymphocytes/*immunology
MH  - Nucleic Acid Conformation
MH  - Nucleic Acids/*immunology
MH  - Oligodeoxyribonucleotides/immunology
MH  - Structure-Activity Relationship
EDAT- 1985/09/01 00:00
MHDA- 1985/09/01 00:01
CRDT- 1985/09/01 00:00
PHST- 1985/09/01 00:00 [pubmed]
PHST- 1985/09/01 00:01 [medline]
PHST- 1985/09/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1985 Sep;135(3):1772-7.