PMID- 4022037
OWN - NLM
STAT- MEDLINE
DCOM- 19850904
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 151
IP  - 1
DP  - 1985 Aug
TI  - DNA damage induced by nitropyrenes in primary mouse hepatocytes and in rat 
      H4-II-E hepatoma cells.
PG  - 137-46
AB  - The capacity of nitropyrenes to cause DNA damage in primary mouse hepatocytes 
      (C57BL/6N mice) and rat H4-II-E hepatoma cells was studied by estimating 
      single-strand breaks using the alkaline elution technique. 1-Nitropyrene (10-200 
      microM) caused clear dose-dependent increases in DNA strand breaks in both cell 
      types, whereas no increase in DNA strand breaks was observed in hepatocytes 
      treated with 1.3-, 1,6-, 1,8-dinitropyrene, 1,3,6-trinitropyrene and 
      1,3,6,8-tetranitropyrene under standard assay conditions (5-20 microM 30-min 
      incubation). However, 1,8-dinitropyrene (1,8-DNP) caused dose-dependent increases 
      in DNA strand breaks when incubated with the H4-II-E cells for 48 h, while no 
      single-strand breaks were observed following treatment with 1,6-dinitropyrene 
      (1,6-DNP) under the same conditions. Neither 1,6-DNP nor 1,8-DNAP induced DNA 
      crosslinks in the H4-II-E cells. These data indicate that substrate specificity 
      exists in the metabolic activation of nitropyrenes in murine liver.
FAU - Moller, M E
AU  - Moller ME
FAU - Thorgeirsson, S S
AU  - Thorgeirsson SS
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Pyrenes)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Cross-Linking Reagents
MH  - DNA/genetics
MH  - Liver/drug effects
MH  - Liver Neoplasms, Experimental/genetics
MH  - Mice
MH  - Mutation/*drug effects
MH  - Pyrenes/*toxicity
MH  - Rats
EDAT- 1985/08/01 00:00
MHDA- 1985/08/01 00:01
CRDT- 1985/08/01 00:00
PHST- 1985/08/01 00:00 [pubmed]
PHST- 1985/08/01 00:01 [medline]
PHST- 1985/08/01 00:00 [entrez]
AID - 0027-5107(85)90192-7 [pii]
AID - 10.1016/0027-5107(85)90192-7 [doi]
PST - ppublish
SO  - Mutat Res. 1985 Aug;151(1):137-46. doi: 10.1016/0027-5107(85)90192-7.