PMID- 4043597
OWN - NLM
STAT- MEDLINE
DCOM- 19851108
LR  - 20190908
IS  - 0272-0590 (Print)
IS  - 0272-0590 (Linking)
VI  - 5
IP  - 4
DP  - 1985 Aug
TI  - In vitro bioassay for dioxinlike activity based on alterations in epithelial cell 
      proliferation and morphology.
PG  - 754-9
AB  - 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) has been shown to induce changes in 
      morphology and proliferation characteristics of a nonkeratinizing derivative 
      (XBF) of a keratinizing epithelial cell line (XB), cloned from a mouse teratoma, 
      when cocultured with irradiated feeder cells. Polychlorinated dibenzodioxins 
      (PCDDs), polychlorinated dibenzofurans (PCDFs), polychlorinated biphenyls (PCBs), 
      polynuclear aromatic hydrocarbons (PAHs), and pesticides (24 compounds in total) 
      were tested for their ability to induce these effects. The results indicated 
      that, for the representative compounds tested, these changes are relatively 
      specific for-and that the XBF cells are extremely sensitive to--PCDDs and PCDFs. 
      TCDD was the most potent congener tested, capable of inducing the effects at a 
      concentration as low as 10(-11) M. The activities of other tested PCDDs and PCDFs 
      ranged from 10(-1) to 10(-3) of TCDD activity. The PCBs, PAHs, and pesticides had 
      lower activities ranging from 10(-3) to 10(-6) that of TCDD. This assay system 
      using XBF cells cocultured with irradiated 3T3 fibroblast feeder cells was 
      examined as a possible in vitro screening assay for dioxinlike activity by 
      testing benzene extracts of soot from a fire involving a PCB-containing 
      transformer. The results were compared to a high-resolution gas 
      chromatographic/mass spectrometric analysis for total PCDFs in the same samples. 
      This comparison showed a good correlation, suggesting that the XBF-3T3 system has 
      potential for use as a semiquantitative assay for dioxinlike activity.
FAU - Gierthy, J F
AU  - Gierthy JF
FAU - Crane, D
AU  - Crane D
LA  - eng
GR  - OHO 1533/OH/NIOSH CDC HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Fundam Appl Toxicol
JT  - Fundamental and applied toxicology : official journal of the Society of 
      Toxicology
JID - 8200838
RN  - 0 (Benzofurans)
RN  - 0 (Dibenzofurans, Polychlorinated)
RN  - 0 (Dioxins)
RN  - 0 (Polychlorinated Dibenzodioxins)
SB  - IM
MH  - Animals
MH  - Benzofurans/analysis
MH  - Biological Assay/*methods
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Dibenzofurans, Polychlorinated
MH  - Dioxins/*analysis
MH  - Epithelium/drug effects/pathology
MH  - Gas Chromatography-Mass Spectrometry
MH  - Mice
MH  - Polychlorinated Dibenzodioxins/analogs & derivatives/*analysis
EDAT- 1985/08/01 00:00
MHDA- 1985/08/01 00:01
CRDT- 1985/08/01 00:00
PHST- 1985/08/01 00:00 [pubmed]
PHST- 1985/08/01 00:01 [medline]
PHST- 1985/08/01 00:00 [entrez]
AID - 10.1016/0272-0590(85)90199-x [doi]
PST - ppublish
SO  - Fundam Appl Toxicol. 1985 Aug;5(4):754-9. doi: 10.1016/0272-0590(85)90199-x.