PMID- 4045848
OWN - NLM
STAT- MEDLINE
DCOM- 19851107
LR  - 20061115
IS  - 0315-162X (Print)
IS  - 0315-162X (Linking)
VI  - 12
IP  - 3
DP  - 1985 Jun
TI  - Sensitive solid phase radioimmunoassay for the detection of anti-DNA 
      autoantibodies.
PG  - 482-6
AB  - Serum from patients with systemic lupus erythematosus (SLE) and hybridoma culture 
      fluids derived from the fusion of SLE lymphocytes contain antibodies to native 
      DNA (nDNA) and denatured DNA (dDNA). A rapid, efficient solid phase 
      radioimmunoassay (RIA) was developed to screen for minute quantities of these 
      autoantibodies. The RIA, which utilized polystyrene tubes, required the addition 
      of 0.1% bovine serum albumin and 0.01% Tween 20 detergent to decrease nonspecific 
      immunoglobulin binding. Pretreatment of the polystyrene tubes with poly-L-lysine 
      (PLL) prior to coating with DNA increased the binding of radiolabeled nDNA from 
      15 to 46% and of dDNA from 17 to 63%. This PLL precoating step resulted in a 
      3-fold increase in the specificity of the assay for nDNA but was not advantageous 
      for dDNA. The method described is sensitive, specific, and can be applied to the 
      screening of microgram quantities of anti-DNA autoantibodies in serum and 
      hybridoma culture fluids.
FAU - Rauch, J
AU  - Rauch J
FAU - Massicotte, H
AU  - Massicotte H
FAU - Wild, J
AU  - Wild J
FAU - Tannenbaum, H
AU  - Tannenbaum H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - J Rheumatol
JT  - The Journal of rheumatology
JID - 7501984
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Autoantibodies)
RN  - 0 (Buffers)
RN  - 0 (Polysorbates)
RN  - 0 (Polystyrenes)
RN  - 25104-18-1 (Polylysine)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Antibodies, Monoclonal/immunology
MH  - Autoantibodies/*analysis
MH  - Buffers
MH  - DNA/*immunology
MH  - Humans
MH  - Lupus Erythematosus, Systemic/*immunology
MH  - Nucleic Acid Denaturation
MH  - Polylysine
MH  - Polysorbates
MH  - Polystyrenes
MH  - Radioimmunoassay/*methods
EDAT- 1985/06/01 00:00
MHDA- 1985/06/01 00:01
CRDT- 1985/06/01 00:00
PHST- 1985/06/01 00:00 [pubmed]
PHST- 1985/06/01 00:01 [medline]
PHST- 1985/06/01 00:00 [entrez]
PST - ppublish
SO  - J Rheumatol. 1985 Jun;12(3):482-6.