PMID- 4052224
OWN - NLM
STAT- MEDLINE
DCOM- 19851219
LR  - 20161020
IS  - 0884-6812 (Linking)
VI  - 7
IP  - 3
DP  - 1985 Sep
TI  - Comparison of cytologic and acridine-orange flow-cytometric detection of 
      malignant cells in human body cavity fluids.
PG  - 227-35
AB  - Flow cytometrically (FCM) derived DNA and RNA profiles were studied in acridine 
      orange (AO)-stained body cavity fluid (BCF) specimens obtained from 78 patients 
      with various solid tissue and hematologic malignancies. The ploidy (DNA index), 
      RNA content (RNA index), proliferative activity (% S + G2M) and DNA and RNA 
      scattergram patterns were tested "double-blind" against the cytologic scoring of 
      specimens as malignant, benign or reactive. It was determined that expression of 
      an "abnormal" RNA index (greater than or equal to 2.8) and an elevated 
      proliferative activity (% S + G2M greater than or equal to 7.4) was dependent on 
      the presence of malignancy; 21 of 22 specimens having those abnormal indices had 
      DNA aneuploidy and were cytologically scored as positive. The AO FCM sensitivity 
      and specificity for detecting malignant cells (when measured against cytology 
      scoring) were 61% and 90%, respectively, using the "abnormal" RNA index and % S + 
      G2M cut-offs together with the cellular DNA aneuploidy marker. By supplementing 
      the cytologic scoring with AO FCM DNA and RNA features, the sensitivity for 
      detecting malignant cells was 94%, as compared to 72% for cytology alone. Two 
      specimens gave false-positive FCM results: a tuberculous effusion with a 
      tetraploid subpopulation and a reactive mesothelial proliferation that was 
      diploid and negative cytologically. Scoring for malignancy based on the visual 
      pattern of the DNA and RNA FCM scattergrams, while showing good correlation for 
      aneuploid specimens, in some cases failed to identify diploid disease. The 
      results demonstrate the usefulness of FCM DNA and RNA analysis for supplementing 
      cytologic examination of BCF specimens for the purpose of detecting malignant 
      cells.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Katz, R L
AU  - Katz RL
FAU - Johnson, T S
AU  - Johnson TS
FAU - Williamson, K D
AU  - Williamson KD
LA  - eng
GR  - CA16672/CA/NCI NIH HHS/United States
GR  - CA28771/CA/NCI NIH HHS/United States
GR  - CA6294/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Anal Quant Cytol Histol
JT  - Analytical and quantitative cytology and histology
JID - 8506819
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Ascites/pathology
MH  - Cytodiagnosis
MH  - *Flow Cytometry
MH  - Humans
MH  - Neoplasms/*diagnosis/genetics
MH  - Pleural Effusion/pathology
MH  - Ploidies
EDAT- 1985/09/01 00:00
MHDA- 1985/09/01 00:01
CRDT- 1985/09/01 00:00
PHST- 1985/09/01 00:00 [pubmed]
PHST- 1985/09/01 00:01 [medline]
PHST- 1985/09/01 00:00 [entrez]
PST - ppublish
SO  - Anal Quant Cytol Histol. 1985 Sep;7(3):227-35.