PMID- 4053403
OWN - NLM
STAT- MEDLINE
DCOM- 19851218
LR  - 20190706
IS  - 0009-8981 (Print)
IS  - 0009-8981 (Linking)
VI  - 152
IP  - 1-2
DP  - 1985 Oct 31
TI  - A simple spectrophotometric method for quantitative fecal carbohydrate 
      measurement.
PG  - 3-9
AB  - A simple quantitative assay was developed for measuring total fecal carbohydrate 
      (CHO) excretion using stools obtained during balance studies performed for fecal 
      fat determination. This spectrophotometric method utilizes Dreywood's anthrone 
      reagent which reacts with equal weights of CHO whether monosaccharide or 
      polysaccharide. Analysis of known dietary CHO solutions yielded greater than 90% 
      of the known theoretical concentration. Recovery of CHO (Polycose) added to fresh 
      stool was greater than 95%, inter-assay coefficient of variation (CV) 6.2%. Stool 
      specimens stored frozen and analyzed over a 21-month period yielded stable 
      results. Fecal CHO excretion/day determined in 32 normal patients, ages 1 month 
      to 13 years, on diets with varying CHO sources, ranged from 0.04 to 0.85 g, 
      average 0.33 g, SD +/- 0.24. Three patients with diseases known to be associated 
      with CHO malabsorption studied showed markedly increased total fecal CHO 
      excretion, up to 53% of their CHO intake. Quantitative fecal CHO excretion in 
      these patients allowed for assessment of the severity of their disease and 
      provided a means for evaluating the use of different dietary CHO sources in their 
      management.
FAU - Ameen, V Z
AU  - Ameen VZ
FAU - Powell, G K
AU  - Powell GK
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Clin Chim Acta
JT  - Clinica chimica acta; international journal of clinical chemistry
JID - 1302422
RN  - 0 (Carbohydrates)
RN  - 0 (Dietary Carbohydrates)
SB  - IM
MH  - Adolescent
MH  - Carbohydrate Metabolism, Inborn Errors/metabolism
MH  - Carbohydrates/*analysis
MH  - Child
MH  - Child, Preschool
MH  - Dietary Carbohydrates/analysis
MH  - Feces/*analysis
MH  - Humans
MH  - Infant
MH  - Spectrophotometry/methods
EDAT- 1985/10/31 00:00
MHDA- 1985/10/31 00:01
CRDT- 1985/10/31 00:00
PHST- 1985/10/31 00:00 [pubmed]
PHST- 1985/10/31 00:01 [medline]
PHST- 1985/10/31 00:00 [entrez]
AID - 0009-8981(85)90169-X [pii]
AID - 10.1016/0009-8981(85)90169-x [doi]
PST - ppublish
SO  - Clin Chim Acta. 1985 Oct 31;152(1-2):3-9. doi: 10.1016/0009-8981(85)90169-x.