PMID- 410889
OWN - NLM
STAT- MEDLINE
DCOM- 19771229
LR  - 20190723
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 17
IP  - 3-4
DP  - 1977
TI  - A rapid micro method for the simultaneous determination of 
      phagocytic-microbiocidal activity of human peripheral blood leukocytes in vitro.
PG  - 241-7
AB  - A new, simple technique for simultaneously studying phagocytic and microbiocidal 
      functions, using viable eukaryotic and prokaryotic microbes, is described. Fresh 
      human venous blood from volunteers was placed on a coverglass and incubated to 
      allow leukocyte adhesion to the coverglass. After clot removal, viable microbes 
      in suspension were added and the coverglass preparation was incubated to allow 
      phagocytosis. The excess microbes (E. coli, S. aureus, L. monocytogenes, and C. 
      albicans each have been used) were then rinsed off, and the vital fluorochrome, 
      acridine orange (AO), was used for staining. A wet mount was prepared and 
      examined by reflected fluorescence with an ultraviolet microscope. Intact 
      (viable) polymorphonuclear (PMN) leukocyte nuclei and microbes appeared green 
      (orthochromatic). Granules in the PMN cytoplasm were yellow or reddish. Nonviable 
      PMN nuclei appeared yellowish or reddish and the nonviable microbes appeared 
      bright red (metachromatic). Thus, phagocytized microbes may be counted and 
      identified as viable or non-viable.
FAU - Smith, D L
AU  - Smith DL
FAU - Rommel, F
AU  - Rommel F
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Acridines)
RN  - I2ZWO3LS3M (Trypan Blue)
SB  - IM
MH  - Acridines/pharmacology
MH  - *Blood Bactericidal Activity
MH  - Candida albicans
MH  - Escherichia coli
MH  - Humans
MH  - Kinetics
MH  - Listeria monocytogenes
MH  - Microchemistry
MH  - Neutrophils/*analysis
MH  - *Phagocytosis
MH  - Staphylococcus aureus
MH  - Time Factors
MH  - Trypan Blue/pharmacology
EDAT- 1977/01/01 00:00
MHDA- 1977/01/01 00:01
CRDT- 1977/01/01 00:00
PHST- 1977/01/01 00:00 [pubmed]
PHST- 1977/01/01 00:01 [medline]
PHST- 1977/01/01 00:00 [entrez]
AID - 0022-1759(77)90106-5 [pii]
AID - 10.1016/0022-1759(77)90106-5 [doi]
PST - ppublish
SO  - J Immunol Methods. 1977;17(3-4):241-7. doi: 10.1016/0022-1759(77)90106-5.