PMID- 4160916
OWN - NLM
STAT- MEDLINE
DCOM- 19660902
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 27
IP  - 2
DP  - 1965 Nov
TI  - Studies on nucleic acid metachromasy. I. The effect of certain fixatives on the 
      dye stacking properties of nucleic acids in solution.
PG  - 313-26
AB  - The stacking coefficients (K's) of nucleic acids have been thought to influence 
      the color contrast between DNA and RNA in tissue sections stained with 
      metachromatic dyes. This idea was tested by titrating toluidine blue (TB) and 
      acridine orange (AO) in solution against DNA and RNA, native or treated with 
      formaldehyde, acrolein, or Carnoy's fluid. Absorption spectra at varying 
      polymer-dye ratios were used to compute K values by the methods of Bradley and 
      colleagues. Results with both dyes fit Bradley's stacking equations. Fixatives 
      did not block dye-binding sites but markedly altered K values. K of DNA was low, 
      unaffected by aldehyde fixative, increased by Carnoy's fluid or heat 
      denaturation. K of RNA was higher than that of DNA and was increased greatly by 
      formaldehyde, almost as much by acrolein, considerably less by Carnoy's fluid. 
      Aldehyde effects were partially reversed upon removal of aldehyde by dialysis. 
      These observations accord with known effects of aldehydes and denaturation upon 
      nucleic acid conformation. Differences between K's of DNA and RNA were greater 
      after aldehyde treatment than after Carnoy's, and were greater with AO than with 
      TB. This is generally consistent with the magnitude of the color contrasts 
      observed in tissues. Additional factors must contribute to the intense color 
      contrast observed in acrolein-fixed tissues stained with TB.
FAU - Lamm, M E
AU  - Lamm ME
FAU - Childers, L
AU  - Childers L
FAU - Wolf, M K
AU  - Wolf MK
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Acetates)
RN  - 0 (Acridines)
RN  - 0 (Aldehydes)
RN  - 0 (Hemostatics)
RN  - 0 (Solutions)
RN  - 1HG84L3525 (Formaldehyde)
RN  - 3K9958V90M (Ethanol)
RN  - 63231-63-0 (RNA)
RN  - 7V31YC746X (Chloroform)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Acetates
MH  - Acridines
MH  - Aldehydes
MH  - Animals
MH  - Cattle
MH  - Chemical Phenomena
MH  - Chemistry, Physical
MH  - Chloroform
MH  - *DNA
MH  - Dialysis
MH  - Ethanol
MH  - Formaldehyde
MH  - Hemostatics
MH  - In Vitro Techniques
MH  - *RNA
MH  - Saccharomyces
MH  - Solutions
MH  - Spectrophotometry
MH  - *Staining and Labeling
MH  - Thymus Gland
PMC - PMC2106731
EDAT- 1965/11/01 00:00
MHDA- 1965/11/01 00:01
PMCR- 1966/05/01
CRDT- 1965/11/01 00:00
PHST- 1965/11/01 00:00 [pubmed]
PHST- 1965/11/01 00:01 [medline]
PHST- 1965/11/01 00:00 [entrez]
PHST- 1966/05/01 00:00 [pmc-release]
AID - 10.1083/jcb.27.2.313 [doi]
PST - ppublish
SO  - J Cell Biol. 1965 Nov;27(2):313-26. doi: 10.1083/jcb.27.2.313.