PMID- 4248448
OWN - NLM
STAT- MEDLINE
DCOM- 19701127
LR  - 20181113
IS  - 0008-4050 (Print)
IS  - 0008-4050 (Linking)
VI  - 34
IP  - 3
DP  - 1970 Jul
TI  - Response of gnotobiotic pigs to Escherichia coli.
PG  - 269-76
AB  - In a study of the response of gnotobiotic pigs to coliform infections, 45 
      one-week-old germfree pigs were divided into five groups and each group was 
      inoculated orally with a different strain of Escherichia coli. Three of these 
      were enteropathogenic swine strains, P307[08:K87(B), K88 a,b (L):H19]; P570 
      [0138:K81]; P568[0141:K85a,b(B), K88a,b(L):H4], one was a virulent human strain, 
      H224, [026:K60(B6)], and one was a non-enteropathogenic swine strain, 
      P581[OX13:K68]. It was attempted to protect a portion of the pigs with orally 
      administered specific antisera and sera from non-immunized specific pathogenfree 
      (SPF) pigs. Observations were made on the clinical response, bacterial counts of 
      feces and intestinal contents, gross pathological changes, distribution of the 
      organisms in organs and serum hemagglutinin titers. Infection with E. coli P307 
      resulted in diarrhea, dehydration and death, unless the pig was protected with 
      specific antiserum. The pigs infected with E. coli P570 had a transient diarrhea 
      but retained their appetites and recovered. Those infected with the other three 
      strains remained healthy throughout. No circulating hemagglutinating antibody 
      against the test strains of E. coli could be detected in any of the pigs seven 
      days or earlier post-inoculation. Relationship could not be established between 
      the numbers of viable E. coli in the feces and the presence of clinical 
      colibacillosis. Orally administered specific antiserum afforded protection 
      against strain P307, but did not reduce the number of E. coli in the gut or alter 
      their distribution in the internal organs. This suggested that the protective 
      effect of specific antibody in the intestine was due to its action on a 
      metabolite (enterotoxin) produced by E. coli P307 rather than the organism 
      itself.
FAU - Miniats, O P
AU  - Miniats OP
FAU - Mitchell, L
AU  - Mitchell L
FAU - Barnum, D A
AU  - Barnum DA
LA  - eng
PT  - Journal Article
PL  - Canada
TA  - Can J Comp Med
JT  - Canadian journal of comparative medicine : Revue canadienne de medecine comparee
JID - 0151747
RN  - 0 (Antibodies)
RN  - 0 (Enterotoxins)
RN  - 0 (Immune Sera)
SB  - IM
MH  - Agglutination Tests
MH  - Animals
MH  - Antibodies
MH  - Bacteriological Techniques
MH  - Enterotoxins/biosynthesis
MH  - Escherichia coli/metabolism
MH  - Escherichia coli Infections/drug therapy/pathology/*veterinary
MH  - Feces/microbiology
MH  - *Germ-Free Life
MH  - Hemagglutination Tests
MH  - Immune Sera/administration & dosage
MH  - Intestines/microbiology
MH  - Swine
MH  - Swine Diseases/drug therapy/*microbiology/pathology
MH  - Virulence
PMC - PMC1319503
EDAT- 1970/07/01 00:00
MHDA- 1970/07/01 00:01
PMCR- 1970/07/01
CRDT- 1970/07/01 00:00
PHST- 1970/07/01 00:00 [pubmed]
PHST- 1970/07/01 00:01 [medline]
PHST- 1970/07/01 00:00 [entrez]
PHST- 1970/07/01 00:00 [pmc-release]
PST - ppublish
SO  - Can J Comp Med. 1970 Jul;34(3):269-76.