PMID- 4263909
OWN - NLM
STAT- MEDLINE
DCOM- 19730118
LR  - 20181113
IS  - 0008-4050 (Print)
IS  - 0008-4050 (Linking)
VI  - 36
IP  - 4
DP  - 1972 Oct
TI  - A comparative study of the rabbit and pig gut loop systems for the assay of 
      Escherichia coli enterotoxin.
PG  - 319-28
AB  - A comparison was made between segments of pig and rabbit small intestine in their 
      response to heat-labile (LT) and heat-stable (ST) preparations from porcine 
      enteropathogenic Escherichia coli. Either whole cell lysates or dialysed broth 
      culture supernatants were used as sources of LT and soft agar culture fluids as a 
      source of ST. Whole cell lysates of all thirteen LT-producing E. coli strains 
      tested regularly elicited fluid accumulation in rabbit gut loops. Whole cell 
      lysates of certain E. coli strains considered to be nonenteropathogenic in pigs 
      could also elicit a positive response in rabbit gut loops. When graded doses of 
      LT were tested in pig and rabbit gut loops, the rabbit was more sensitive and is 
      therefore considered preferable to the pig for quantitation of LT. In the rabbit, 
      upper (jejunal) and lower (ileal) small intestine were compared for their 
      response to LT and it was found that ileal loops were twice as sensitive but more 
      prone to false positive reactions. When soft agar culture fluids of several 
      enteropathogenic E. coli strains were tested in the rabbit, the response was 
      inconsistent, and it was concluded that the rabbit is unsuitable for the assay of 
      the heat-stable enterotoxin.
FAU - Lariviere, S
AU  - Lariviere S
FAU - Gyles, C L
AU  - Gyles CL
FAU - Barnum, D A
AU  - Barnum DA
LA  - eng
PT  - Journal Article
PL  - Canada
TA  - Can J Comp Med
JT  - Canadian journal of comparative medicine : Revue canadienne de medecine comparee
JID - 0151747
RN  - 0 (Culture Media)
RN  - 0 (Enterotoxins)
SB  - IM
MH  - Animals
MH  - *Biological Assay
MH  - Cell-Free System
MH  - Culture Media
MH  - Enterotoxins/*analysis
MH  - Escherichia coli/analysis/growth & development/isolation & purification
MH  - Escherichia coli Infections/diagnosis/*veterinary
MH  - Hot Temperature
MH  - Ileum/drug effects
MH  - Intestine, Small/*drug effects
MH  - Jejunum/drug effects
MH  - Methods
MH  - Rabbits
MH  - Swine
MH  - Swine Diseases/*diagnosis
PMC - PMC1319692
EDAT- 1972/10/01 00:00
MHDA- 1972/10/01 00:01
PMCR- 1972/10/01
CRDT- 1972/10/01 00:00
PHST- 1972/10/01 00:00 [pubmed]
PHST- 1972/10/01 00:01 [medline]
PHST- 1972/10/01 00:00 [entrez]
PHST- 1972/10/01 00:00 [pmc-release]
PST - ppublish
SO  - Can J Comp Med. 1972 Oct;36(4):319-28.