PMID- 4287276
OWN - NLM
STAT- MEDLINE
DCOM- 19660925
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 27
IP  - 3
DP  - 1965 Dec
TI  - Ribonucleic acid and protein synthesis in mitotic HeLa cells.
PG  - 565-74
AB  - HeLa cells arrested in mitosis were obtained in large numbers, with only very 
      slight interphase cell contamination, by employing the agitation method of 
      Terasima and Tolmach, and Robbins and Marcus. Protein synthesis and RNA synthesis 
      were almost completely suppressed in mitotic cells. Active polyribosomes were 
      nearly absent in mitotic cells as compared with interphase cells treated in the 
      same way. Cell-free protein synthesis and RNA polymerase activity were also 
      greatly depressed in extracts of metaphase cells. The deoxyribonucleoprotein 
      (DNP) of condensed chromosomes from mitotic cells was less efficient as a 
      template for Escherichia coli RNA polymerase than was DNP from interphase cells, 
      although isolated DNA from both sources was equally active as a primer. Despite 
      very poor endogenous amino acid incorporation by extracts of metaphase cells, 
      polyuridylate stimulated phenylalanine incorporation by a larger factor in 
      mitotic cell extracts than it did in interphase cell extracts. These results 
      suggest that RNA synthesis is suppressed in mitotic cells because the condensed 
      chromosomes cannot act as a template, and that protein synthesis is depressed at 
      least in part because messenger RNA becomes unavailable to ribosomes. This 
      conclusion was supported by the demonstration that cells arrested in metaphase 
      supported multiplication of normal yields of poliovirus, thereby showing that the 
      mitotic cell is capable of considerable synthesis of RNA and protein.
FAU - Johnson, T C
AU  - Johnson TC
FAU - Holland, J J
AU  - Holland JJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 47E5O17Y3R (Phenylalanine)
RN  - 5V9KLZ54CY (Vinblastine)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.7.- (Nucleotidyltransferases)
SB  - IM
MH  - *Cell Division
MH  - *Culture Techniques
MH  - HeLa Cells
MH  - Nucleotidyltransferases/metabolism
MH  - Phenylalanine/metabolism
MH  - Poliovirus
MH  - *Protein Biosynthesis
MH  - RNA/*biosynthesis
MH  - Ribosomes
MH  - Vinblastine/pharmacology
PMC - PMC2106774
EDAT- 1965/12/01 00:00
MHDA- 1965/12/01 00:01
PMCR- 1966/06/01
CRDT- 1965/12/01 00:00
PHST- 1965/12/01 00:00 [pubmed]
PHST- 1965/12/01 00:01 [medline]
PHST- 1965/12/01 00:00 [entrez]
PHST- 1966/06/01 00:00 [pmc-release]
AID - 10.1083/jcb.27.3.565 [doi]
PST - ppublish
SO  - J Cell Biol. 1965 Dec;27(3):565-74. doi: 10.1083/jcb.27.3.565.