PMID- 429199
OWN - NLM
STAT- MEDLINE
DCOM- 19790611
LR  - 20200304
IS  - 0018-2214 (Print)
IS  - 0018-2214 (Linking)
VI  - 11
IP  - 1
DP  - 1979 Jan
TI  - The ultrastructural localization of the enzymes related to steroid hormone 
      metabolism in the guinea-pig testis.
PG  - 51-71
AB  - A study of the ultrastructural localization of 3 beta-hydroxysteroid 
      dehydrogenase (3 beta-HSD), 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD), 
      glucose-6-phosphate dehydrogenase (G-6-PD), beta-hydroxybutyrate dehydrogenase 
      (beta-HBD), NADH diaphorase (NADH-D) and NADPH diaphorase (NADPH-D) in the 
      guinea-pig testis is reported. The procedures employed included short immersion 
      or perfusion fixation with aldehydes followed by incubation of small blocks in a 
      tetrazolium salt or a ferricyanide medium. The effects of incubation conditions 
      were investigated, and a reaction medium for the ultracytochemical demonstration 
      of 11 beta-HSD is described. Using suitable controls, evidence for the 
      specificity of the cytochemical reactions is presented. It was found that all the 
      enzymes studied were present in both the Leydig and Sertoli cells of the 
      guinea-pig testis and that the intracellular distribution pattern for each enzyme 
      was independent of the cell type. Using tetrazolium salt techniques, both 3 
      beta-HSD and 11 beta-HSD activities were localized on or in membranes of smooth 
      endoplasmic reticulum and within the mitochondria. With the ferricyanide 
      techniques, G-6-PD activity was found to be associated mainly with the smooth 
      endoplasmic reticulum membranes, while beta-HBD activity was limited to 
      mitochondria. With both the tetrazolium salt and ferricyanide techniques, the 
      reaction products for NADH-D and NADPH-D activities showed localizations which 
      were similar to those observed for the steroid dehydrogenases.
FAU - Bara, G
AU  - Bara G
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Histochem J
JT  - The Histochemical journal
JID - 0163161
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.30 (Hydroxybutyrate Dehydrogenase)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 1.6.99.1 (NADPH Dehydrogenase)
RN  - EC 1.8.1.4 (Dihydrolipoamide Dehydrogenase)
SB  - IM
MH  - Animals
MH  - Dihydrolipoamide Dehydrogenase/*isolation & purification
MH  - Endoplasmic Reticulum/enzymology
MH  - Glucosephosphate Dehydrogenase/*isolation & purification
MH  - Guinea Pigs
MH  - Hydroxybutyrate Dehydrogenase/*isolation & purification
MH  - Hydroxysteroid Dehydrogenases/*isolation & purification
MH  - Leydig Cells/enzymology
MH  - Male
MH  - Mitochondria/enzymology
MH  - NADPH Dehydrogenase/*isolation & purification
MH  - Sertoli Cells/enzymology
MH  - Testis/*enzymology
EDAT- 1979/01/01 00:00
MHDA- 1979/01/01 00:01
CRDT- 1979/01/01 00:00
PHST- 1979/01/01 00:00 [pubmed]
PHST- 1979/01/01 00:01 [medline]
PHST- 1979/01/01 00:00 [entrez]
AID - 10.1007/BF01041265 [doi]
PST - ppublish
SO  - Histochem J. 1979 Jan;11(1):51-71. doi: 10.1007/BF01041265.