PMID- 440966
OWN - NLM
STAT- MEDLINE
DCOM- 19790716
LR  - 20190501
IS  - 0305-1048 (Print)
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 6
IP  - 3
DP  - 1979 Mar
TI  - The DNA polymerases associated with the adenovirus type 2 replication complex: 
      effect of 2'-3'-dideoxythymidine-5'-triphosphate on viral DNA synthesis.
PG  - 1025-39
AB  - An adenovirus (Ad) DNA replication complex extracted from infected HeLa nuclei 
      could be purified free of the bulk of intracellular DNA polymerase activity by 
      sedimetation in neutral sucrose gradients. However, the replication complex still 
      retained some alpha and gamma DNA-polymerase activity. Since this complex is 
      inhibited by 2', 3' dideoxythymidine-5'-triphosphate (ddTTP), an inhibitor of DNA 
      polymerase gamma, a functional role for this enzyme in Ad DNA replication is 
      suggested. Similar inhibition by ddTTP in intact Ad infected nuclei and 
      comparable inhibition of Ad DNA synthesis in whole cells by dideoxythymidine 
      (ddThy) are consistent with a role for DNA polymerase gamma. Uninfected HeLa 
      nuclei or whole cells are not similarly inhibited by ddTTP or DDThy respectively. 
      Such data does not rule out an additional functional role for other DNA 
      polymerases, and recent experiments from this laboratory (1) suggest that DNA 
      polymerase alpha is also involved in Ad DNA synthesis.
FAU - Abboud, M M
AU  - Abboud MM
FAU - Horwitz, M S
AU  - Horwitz MS
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (DNA, Viral)
RN  - 0 (Thymine Nucleotides)
RN  - EC 2.7.7.7 (DNA-Directed DNA Polymerase)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Adenoviruses, Human/*enzymology
MH  - Cell Nucleus/enzymology
MH  - *DNA Replication
MH  - DNA, Viral/*biosynthesis
MH  - DNA-Directed DNA Polymerase/isolation & purification/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Kinetics
MH  - Thymidine/analogs & derivatives/pharmacology
MH  - Thymine Nucleotides/*pharmacology
MH  - Virus Replication
PMC - PMC327750
EDAT- 1979/03/01 00:00
MHDA- 1979/03/01 00:01
CRDT- 1979/03/01 00:00
PHST- 1979/03/01 00:00 [pubmed]
PHST- 1979/03/01 00:01 [medline]
PHST- 1979/03/01 00:00 [entrez]
AID - 10.1093/nar/6.3.1025 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1979 Mar;6(3):1025-39. doi: 10.1093/nar/6.3.1025.