PMID- 4577415
OWN - NLM
STAT- MEDLINE
DCOM- 19730910
LR  - 20210526
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 7
IP  - 6
DP  - 1973 Jun
TI  - Differences in the response of rabbit small intestine to heat-labile and 
      heat-stable enterotoxins of Escherichia coli.
PG  - 873-80
AB  - The response of adult rabbit small intestine to the heat-stable (ST) and 
      heat-labile (LT) enterotoxins of Escherichia coli has been investigated by 
      employing the ligated loop technique. Fluid accumulation was determined in 
      relation to enterotoxin dose and duration of gut exposure. The individual 
      responses to ST and LT differed in a characteristic manner. Onset of net fluid 
      accumulation in response to ST appeared to be immediate even at the lowest dose 
      tested. Onset of net fluid accumulation in response to LT was rapid at high doses 
      but delayed at low doses. Maximum volume per length ratios elicited by ST 
      occurred between 4 and 6 h after injection of loops over the entire range of 
      doses tested. However, maximum ratios elicited by LT occurred no less than 10 h 
      after injection even at low doses. Fluid accumulation elicited by LT increased in 
      duration with increasing dose; high doses of LT producing a response which was 
      sustained for at least 18 h. The net effect of these differences in reaction 
      characteristics is a sharp increase in the proportion of the cumulative net 
      secretory response attributable to LT with time. Therefore, a 6-h assay time is 
      appropriate for the titration of ST, whereas an 18-h assay is not. The 18-h assay 
      was found more appropriate for toxin-antitoxin neutralization studies since only 
      LT was neutralized by anti-enterotoxin serum. LT of E. coli (swine) strain P-263 
      and (human) strain 334 was neutralized by antibody stimulated by enterotoxin from 
      E. coli (human) strain H-10407. LT was labile to mild acid conditions, whereas ST 
      was not.
FAU - Evans, D G
AU  - Evans DG
FAU - Evans, D J Jr
AU  - Evans DJ Jr
FAU - Pierce, N F
AU  - Pierce NF
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Enterotoxins)
RN  - 0 (Immune Sera)
SB  - IM
MH  - Animals
MH  - Enterotoxins/*pharmacology
MH  - *Escherichia coli
MH  - *Hot Temperature
MH  - Hydrogen-Ion Concentration
MH  - Immune Sera
MH  - Intestinal Secretions/*drug effects
MH  - Intestine, Small/*drug effects/metabolism
MH  - Kinetics
MH  - Neutralization Tests
MH  - Rabbits
MH  - Time Factors
PMC - PMC422777
EDAT- 1973/06/01 00:00
MHDA- 2001/03/28 10:01
PMCR- 1973/06/01
CRDT- 1973/06/01 00:00
PHST- 1973/06/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1973/06/01 00:00 [entrez]
PHST- 1973/06/01 00:00 [pmc-release]
AID - 10.1128/iai.7.6.873-880.1973 [doi]
PST - ppublish
SO  - Infect Immun. 1973 Jun;7(6):873-80. doi: 10.1128/iai.7.6.873-880.1973.