PMID- 477403
OWN - NLM
STAT- MEDLINE
DCOM- 19791121
LR  - 20180214
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 24
IP  - 2
DP  - 1979
TI  - Regional assignment of human genes TPI1, GAPDH, LDHB, SHMT, and PEPB on 
      chromosome 12.
PG  - 102-14
AB  - Karyological analysis was performed on a series of human-Chinese hamster cell 
      hybrids containing deletions of human chromosome 12. Chromosome breakage was 
      produced by treatment of the cells with either X-rays or 5-bromodeoxyuridine and 
      near-visible light. The hybrid clones were analyzed for the presence or absence 
      of the following five human gene markers known to be located on chromosome 12: 
      triosephosphate isomerase-1 (TPI1), glyceraldehyde-3-phosphate dehydrogenase 
      (GAPDH), lactate dehydrogenase-B (LDHB), serine hydroxymethyltransferase (SHMT), 
      and peptidase-B (PEPB). Based on the correlation between the isozyme markers and 
      karyological analysis of these clones, a regional map of the five human genes on 
      chromosome 12 was established. The linear order for these genes is: 
      pter-TPI1-GAPDH-LDHB-centromere-SHMT-PEPB-qter. The locations of these genes are: 
      TPI1, GAPDH, LDHB: pter leads to p12; SHMT: q12 leads to q14; PEPB: q14 leads to 
      qter. Statistical analysis similar to that of Goss and Harris (1975, 1977a, b) 
      has been performed on the segregation data in the hybrid clones. The statistical 
      map, in general, agrees with the cytogenetic map and further localizes PEPB to 
      12q21.
FAU - Law, M L
AU  - Law ML
FAU - Kao, F T
AU  - Kao FT
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - EC 2.1.2.1 (Glycine Hydroxymethyltransferase)
RN  - EC 3.4.11.1 (Leucyl Aminopeptidase)
RN  - EC 5.3.1.1 (Triose-Phosphate Isomerase)
SB  - IM
MH  - Animals
MH  - Chromosome Aberrations
MH  - *Chromosome Mapping
MH  - *Chromosomes, Human, 6-12 and X
MH  - Clone Cells/enzymology
MH  - Cricetinae
MH  - Cricetulus
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/genetics
MH  - Glycine Hydroxymethyltransferase/genetics
MH  - Humans
MH  - Hybrid Cells/enzymology
MH  - L-Lactate Dehydrogenase/genetics
MH  - Leucyl Aminopeptidase/genetics
MH  - Triose-Phosphate Isomerase/genetics
EDAT- 1979/01/01 00:00
MHDA- 1979/01/01 00:01
CRDT- 1979/01/01 00:00
PHST- 1979/01/01 00:00 [pubmed]
PHST- 1979/01/01 00:01 [medline]
PHST- 1979/01/01 00:00 [entrez]
AID - 10.1159/000131363 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1979;24(2):102-14. doi: 10.1159/000131363.