PMID- 479614
OWN - NLM
STAT- MEDLINE
DCOM- 19791121
LR  - 20190723
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 29
IP  - 2
DP  - 1979
TI  - An enzyme-linked immunosorbent assay for antibodies to native and denatured DNA.
PG  - 155-65
AB  - A modification of the enzyme-linked immunosorbent assay (ELISA) is described, 
      that permits determination of antibodies to native DNA (nDNA). The same approach 
      can be used to measure antibodies to denatured DNA (dDNA). Poor binding of nDNA 
      to the polystyrene solid phase has presented difficulties in using the ELISA 
      method for assaying anti-nDNA activity (Engvall, 1976), but we find that 
      precoating of the solid phase with protamine sulfate circumvents this problem. 
      Assays for anti-dDNA are also enhanced by the use of protamine sulfate coated 
      tubes. We have used the ELISA method to assay 15 SLE and 27 non-SLE sera for 
      anti-nDNA and anti-dDNA activity. The results are compared with those obtained 
      using the GF/A glass fiber filter assay, previously described by Lewis et al. 
      (1973).
FAU - Klotz, J L
AU  - Klotz JL
FAU - Minami, R M
AU  - Minami RM
FAU - Teplitz, R L
AU  - Teplitz RL
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Antibodies)
RN  - 0 (Protamines)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - *Antibodies
MH  - Binding Sites
MH  - DNA/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Filtration/instrumentation
MH  - Glass
MH  - Goats
MH  - Humans
MH  - Lupus Erythematosus, Systemic/immunology
MH  - Protamines/pharmacology
EDAT- 1979/01/01 00:00
MHDA- 1979/01/01 00:01
CRDT- 1979/01/01 00:00
PHST- 1979/01/01 00:00 [pubmed]
PHST- 1979/01/01 00:01 [medline]
PHST- 1979/01/01 00:00 [entrez]
AID - 0022-1759(79)90065-6 [pii]
AID - 10.1016/0022-1759(79)90065-6 [doi]
PST - ppublish
SO  - J Immunol Methods. 1979;29(2):155-65. doi: 10.1016/0022-1759(79)90065-6.