PMID- 4852192
OWN - NLM
STAT- MEDLINE
DCOM- 19741111
LR  - 20210526
IS  - 0003-6919 (Print)
IS  - 0003-6919 (Linking)
VI  - 28
IP  - 2
DP  - 1974 Aug
TI  - Growth rates of a pseudomonad on 2,4-dichlorophenoxyacetic acid and 
      2,4-dichlorophenol.
PG  - 181-4
AB  - The growth of a pseudomonad on 2,4-D (2,4-dichlorophenoxyacetic acid) and 2,4-DCP 
      (2,4-dichlorophenol) was studied in batch and continuous culture. The optimum 
      growth rate using 2,4-D was 0.14/h at 25 C in a pH range from 6.2 to 6.9. Highest 
      specific growth rate using 2,4-DCP was 0.12/h at 25 C in a pH range from 7.1 to 
      7.8. Growth was strongly inhibited by 2,4-DCP above a concentration of 25 
      mg/liter whereas no appreciable inhibition was observed with 2,4-D at 
      concentrations up to 2,000 mg per liter. Growth on 2,4-DCP was described by Monod 
      kinetics at subinhibitory concentrations but the inhibition by 2,4-DCP exhibited 
      an unusual linear response to substrate concentration, and did not fit a model 
      based on noncompetitive inhibition. The lag phase of batch cultures was found to 
      depend on both 2,4-DCP concentration and prior adaptation of the inoculum. A 
      study such as this on the kinetics of growth on related substrates may be useful 
      as a method of finding the rate-limiting step in a metabolic sequence.
FAU - Tyler, J E
AU  - Tyler JE
FAU - Finn, R K
AU  - Finn RK
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Appl Microbiol
JT  - Applied microbiology
JID - 7605802
RN  - 0 (Industrial Waste)
RN  - 0 (Phenols)
RN  - 2577AQ9262 (2,4-Dichlorophenoxyacetic Acid)
SB  - IM
MH  - 2,4-Dichlorophenoxyacetic Acid/*pharmacology
MH  - Fluorometry
MH  - Hydrogen-Ion Concentration
MH  - *Industrial Waste
MH  - Phenols/*pharmacology
MH  - Pseudomonas/drug effects/*growth & development
MH  - Temperature
MH  - *Water Microbiology
PMC - PMC186683
EDAT- 1974/08/01 00:00
MHDA- 1974/08/01 00:01
PMCR- 1974/08/01
CRDT- 1974/08/01 00:00
PHST- 1974/08/01 00:00 [pubmed]
PHST- 1974/08/01 00:01 [medline]
PHST- 1974/08/01 00:00 [entrez]
PHST- 1974/08/01 00:00 [pmc-release]
AID - 10.1128/am.28.2.181-184.1974 [doi]
PST - ppublish
SO  - Appl Microbiol. 1974 Aug;28(2):181-4. doi: 10.1128/am.28.2.181-184.1974.