PMID- 4855738 OWN - NLM STAT- MEDLINE DCOM- 19740322 LR - 20200724 IS - 0022-538X (Print) IS - 1098-5514 (Electronic) IS - 0022-538X (Linking) VI - 13 IP - 2 DP - 1974 Feb TI - Replication of reticuloendotheliosis viruses in cell culture: acute infection. PG - 291-7 AB - Replication of reticuloendotheliosis viruses (REV) in cultures of chicken and duck fibroblasts leads to some cell death soon after infection. This cell killing was used to develop a plaque assay for Trager duck spleen necrosis virus (TDSNV) on duck embryo fibroblasts. A normal replicative cell cycle was required for normal virus production and the development of cytopathic effects in chicken cells exposed to TDSNV. The latent period was about two days. Stationary chicken embryo fibroblasts could be infected by REV; DNA synthesis was required, but protein synthesis was not. FAU - Temin, H M AU - Temin HM FAU - Kassner, V K AU - Kassner VK LA - eng PT - Journal Article PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Immune Sera) RN - 0 (Mitomycins) RN - 04079A1RDZ (Cytarabine) RN - 10028-17-8 (Tritium) RN - 9007-49-2 (DNA) RN - 98600C0908 (Cycloheximide) RN - EC 2.7.7.- (DNA Nucleotidyltransferases) RN - SML2Y3J35T (Colchicine) RN - VC2W18DGKR (Thymidine) SB - IM MH - Animals MH - Cattle/immunology MH - Cell Division/drug effects MH - Cell-Free System MH - Chick Embryo MH - Colchicine/pharmacology MH - Culture Techniques MH - Cycloheximide/pharmacology MH - Cytarabine/pharmacology MH - Cytopathogenic Effect, Viral MH - DNA/biosynthesis MH - DNA Nucleotidyltransferases/metabolism MH - Ducks MH - Fibroblasts MH - Immune Sera MH - Mitomycins/pharmacology MH - Oncogenic Viruses/*growth & development MH - Poultry Diseases/*microbiology MH - Protein Biosynthesis MH - RNA Viruses/enzymology/*growth & development MH - Reticuloendotheliosis, Avian/microbiology/*veterinary MH - Thymidine/metabolism MH - Tritium MH - Viral Plaque Assay MH - Virus Replication/*drug effects PMC - PMC355296 EDAT- 1974/02/01 00:00 MHDA- 1974/02/01 00:01 PMCR- 1974/02/01 CRDT- 1974/02/01 00:00 PHST- 1974/02/01 00:00 [pubmed] PHST- 1974/02/01 00:01 [medline] PHST- 1974/02/01 00:00 [entrez] PHST- 1974/02/01 00:00 [pmc-release] AID - 10.1128/JVI.13.2.291-297.1974 [doi] PST - ppublish SO - J Virol. 1974 Feb;13(2):291-7. doi: 10.1128/JVI.13.2.291-297.1974.