PMID- 497268
OWN - NLM
STAT- MEDLINE
DCOM- 19800119
LR  - 20181130
IS  - 0320-9725 (Print)
IS  - 0320-9725 (Linking)
VI  - 44
IP  - 7
DP  - 1979 Jul
TI  - [Optimization of isolation conditions for three types of pigment protein-lipid 
      complexes from chloroplasts during solubilization with Triton X-100].
PG  - 1160-71
AB  - The maximal total release of pigment protein-lipid complexes (PPLC) during their 
      isolation from pea chloroplasts was achieved by 1-hr solubilization with Triton 
      X-100, the Triton:chlorophyll (T/Chl) ratio being 50 mg/mg/ml. The total yield of 
      the reaction center complexes (sigma PPLC RC) was 22,3%, whereas that of the 
      auxiliary light-accumulating complex (ALA-PPLC) was approximately 32% with 
      respect to Chl. An increase in the solubilization time and of the T/Chl ratio 
      resulted in dissociation of ALA-PPLC. On the contrary, the reaction center 
      complexes steadily maintained their composition and high photochemical activity 
      within a wide range of T/Chl during 24--28 hrs of solubilization. The purest 
      preparations of PPLC RC of phostosystem I (PS-I) were obtained by 24 
      hr-incubation (T/Chl = 80); their Chl/P700 ratio after a single fractionation on 
      DEAE-cellulose was equal to 36. A considerable increase of T/Chl and of the 
      solubilization time hampered the chromatographical separation of PPLC RC of PS-I 
      and PPLC RC of PS-II. The optimal conditions for isolation of PPLC RC of PS-I and 
      PPLC RC of PS-II were: solubilization at T/Chl 80--120 and prolongation of 
      incubation time from 5 to 7 hrs. The photochemical activity of the complexes 
      obtained was maximal and correlated with the minimal content of admixture P700 (1 
      molecule of P700 per 450--500 molecules of Chl.).
FAU - Shutilova, N I
AU  - Shutilova NI
FAU - Kadoshnikova, I G
AU  - Kadoshnikova IG
FAU - Kozlovskaia, N G
AU  - Kozlovskaia NG
FAU - Klevanik, A V
AU  - Klevanik AV
FAU - Zakrzhevskii, D A
AU  - Zakrzhevskii DA
LA  - rus
PT  - Journal Article
TT  - Optimizatsiia uslovii vydeleniia trekh tipov pigment-belkovolipidnykh kompleksov 
      khloroplastov gorokha pri soliubilizatsii s pomoshch'iu tritona X-100.
PL  - Russia (Federation)
TA  - Biokhimiia
JT  - Biokhimiia (Moscow, Russia)
JID - 0372667
RN  - 0 (Lipids)
RN  - 0 (Pigments, Biological)
RN  - 0 (Plant Proteins)
RN  - 1406-65-1 (Chlorophyll)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
SB  - IM
MH  - Chlorophyll/*isolation & purification
MH  - Chloroplasts/*analysis
MH  - Light
MH  - Lipids/*isolation & purification
MH  - Methods
MH  - Pigments, Biological/*isolation & purification
MH  - Plant Proteins/*isolation & purification
MH  - Plants
MH  - Polyethylene Glycols
MH  - Solubility
MH  - Spectrophotometry
EDAT- 1979/07/01 00:00
MHDA- 1979/07/01 00:01
CRDT- 1979/07/01 00:00
PHST- 1979/07/01 00:00 [pubmed]
PHST- 1979/07/01 00:01 [medline]
PHST- 1979/07/01 00:00 [entrez]
PST - ppublish
SO  - Biokhimiia. 1979 Jul;44(7):1160-71.