PMID- 4990553
OWN - NLM
STAT- MEDLINE
DCOM- 19701206
LR  - 20181113
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 19
IP  - 3
DP  - 1970 Sep
TI  - A study of the heterogeneity of anti-dinitrophenyl (DNP) antibodies using 
      inhibition of bacteriophage neutralization.
PG  - 429-37
AB  - The dinitrophenyl (DNP) determinant was conjugated to T2 bacteriophage to yield 
      conjugated phage (DNP-phage). Plaque formation by the DNP-phage was neutralized 
      by rabbit anti-DNP antibodies. This neutralization was inhibited by incubating 
      the antibody first with free hapten and subsequently with the DNP-phage. Both 
      DNP-lysine and dinitro-aniline (DN-aniline) inhibited the neutralization of 
      plaque formation. With antibodies obtained 8 days after immunization there was 
      little difference between the inhibitory capacity of DNP-lysine and that of 
      DN-aniline. However, with antibodies obtained 3 weeks after primary immunization, 
      the inhibitory capacity of DNP-lysine was approximately three times greater than 
      that of DN-aniline. The difference between the inhibitory capacity of the two 
      haptens was further increased with respect to antibodies obtained 8 weeks after 
      primary and 4 weeks after secondary immunization. With the latter two 
      preparations of antibodies, DNP-lysine was approximately ten time more effective 
      than DN-aniline. With antibody preparations from all but the earliest bleedings, 
      the difference between the neutralization inhibitory capacity of DNP-lysine and 
      DN-aniline depended on antibody concentration: at lower concentrations of 
      antibodies the difference between the two inhibitors was at a minimum and 
      increased as the antibody concentration increased. This variation in the relative 
      capacity of the two haptens to inhibit neutralization is attributed to the 
      heterogeneity of the anti-DNP antibodies.
FAU - Carter, B G
AU  - Carter BG
LA  - eng
PT  - Journal Article
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Aniline Compounds)
RN  - 0 (Antibodies)
RN  - 0 (Dinitrophenols)
RN  - 0 (Haptens)
RN  - K3Z4F929H6 (Lysine)
SB  - IM
MH  - Aniline Compounds
MH  - Animals
MH  - *Antibodies
MH  - Cattle
MH  - Coliphages/*immunology
MH  - *Dinitrophenols
MH  - Haptens
MH  - Immunodiffusion
MH  - Lysine
MH  - *Neutralization Tests
MH  - Rabbits
PMC - PMC1455683
EDAT- 1970/09/01 00:00
MHDA- 1970/09/01 00:01
PMCR- 1971/09/01
CRDT- 1970/09/01 00:00
PHST- 1970/09/01 00:00 [pubmed]
PHST- 1970/09/01 00:01 [medline]
PHST- 1970/09/01 00:00 [entrez]
PHST- 1971/09/01 00:00 [pmc-release]
PST - ppublish
SO  - Immunology. 1970 Sep;19(3):429-37.