PMID- 503851
OWN - NLM
STAT- MEDLINE
DCOM- 19800128
LR  - 20190501
IS  - 0305-1048 (Print)
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 7
IP  - 4
DP  - 1979 Oct 25
TI  - Sub-genomic RNA in Moloney leukemia virus grown in lymphoid-derived cell lines 
      consists primarily of homologous viral RNA.
PG  - 1091-108
AB  - Moloney murine leukemia virus (MoMuLV) grown in 2 lymphoid-derived murine cell 
      lines (JLS-V9 and TB) contained 2 size classes of RNA subunits: 2.8 +/- 0.4 x 
      10(6) (n = 30) and 1.6 +/- 0.1 x 10(6) (n = 15) daltons. Detectable levels of low 
      molecular weight viral RNA (LMW vRNA) were not present in MoMuLV grown in mouse 
      embryo fibroblasts, nor rat cells, nor was it produced by them when they were 
      infected with MoMuLV containing both species of vRNA. DNA probes complementary to 
      both subunits were synthesized separately using purified reverse transcriptase 
      (R.T.) and calf thymus DNA as primer. Polyadenylated LMW vRNA was selected and, 
      by molecular hybridization was found to be completely homologous with the large 
      RNA subunit. This was confirmed using cDNA probes representing defined regions of 
      the genome. The LMW vRNA therefore contains multiple subsets of the viral RNA, 
      which could correspond to multiple deletion mutants possibly generated by an 
      efficient RNA splicing mechanism. In addition, low levels of non-homologous 
      virus-like RNA were detected in MoMuLV grown in TB (2%) AND NIH/3T3 cells (0.4%).
FAU - Ball, J K
AU  - Ball JK
FAU - Dekaban, G A
AU  - Dekaban GA
FAU - Loosmore, S M
AU  - Loosmore SM
FAU - Chan, S K
AU  - Chan SK
FAU - McCarter, J A
AU  - McCarter JA
LA  - eng
PT  - Journal Article
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Macromolecular Substances)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Base Sequence
MH  - Genes, Viral
MH  - Kinetics
MH  - Macromolecular Substances
MH  - Molecular Weight
MH  - Moloney murine leukemia virus/*analysis
MH  - Nucleic Acid Hybridization
MH  - RNA, Viral/*analysis
PMC - PMC342285
EDAT- 1979/10/25 00:00
MHDA- 1979/10/25 00:01
CRDT- 1979/10/25 00:00
PHST- 1979/10/25 00:00 [pubmed]
PHST- 1979/10/25 00:01 [medline]
PHST- 1979/10/25 00:00 [entrez]
AID - 10.1093/nar/7.4.1091 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1979 Oct 25;7(4):1091-108. doi: 10.1093/nar/7.4.1091.