PMID- 51109
OWN - NLM
STAT- MEDLINE
DCOM- 19751204
LR  - 20190918
IS  - 0021-5112 (Print)
IS  - 0021-5112 (Linking)
VI  - 28
IP  - 1
DP  - 1975 Feb
TI  - Cell-mediated and humoral immunity in mice: cross reaction between lysozyme and 
      S-carboxymethylated lysozyme studied by a modified footpad test.
PG  - 23-35
AB  - The mouse sensitized by subcutaneous (sc) injection of lysozyme in emulsion of 
      Freund's complete adjuvant (FCA) was shown by a modified footpad test to develop 
      three kinds of hypersensitivities. Injecting lysozyme in 2.5-mul emulsion of 
      Freund's incomplete adjuvant (FIA) into the footpad elicited strong footpad 
      swelling in 30 min (anaphylactic reaction), in 3 hr (Arthus-type reaction) and in 
      24 hr (delayed-type hypersensitivity; DTH). The mice showing anaphylactic 
      reaction in the footpad test manifested severe active systemic anaphylaxis, and 
      the sera of these animals showed high IgG1 antibody titers with only sparingly 
      detectable or no IgE antibody titers. In the sensitizing system with the use of 
      FCA, the antigenicity of S-carboxymethylated lysozyme (CM-lysozyme) devoid of the 
      three-dimensional conformation of lysozyme was compared with that of the native 
      molecule. CM-lysozyme and lysozyme completely cross-reacted to each other in DTH, 
      but not at all in the anaphylactic or Arthus-type reaction or in IgG1 antibody 
      production. CM-lysozyme was shown also to have the ability to bestow 
      immunological memory for the induction of humoral immunity against lysozyme; 
      intravenous (iv) injection of lysozyme in saline or sc injection of 
      CM-lysozyme-FCA alone failed to induce immediate hypersensitivities and IgG1 
      antibody production against lysozyme, but pre-sensitization by sc injection of 
      CM-lysozyme-FCA enabled the animal to induce these responses to significant 
      levels when iv injection of lysozyme in saline was given as a booster.
FAU - Sugimoto, M
AU  - Sugimoto M
FAU - Kojima, A
AU  - Kojima A
FAU - Yaginuma, K
AU  - Yaginuma K
FAU - Egashira, Y
AU  - Egashira Y
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Japan
TA  - Jpn J Med Sci Biol
JT  - Japanese journal of medical science & biology
JID - 0243706
RN  - 0 (Carboxylic Acids)
RN  - 0 (Epitopes)
RN  - 0 (Immunoglobulin G)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.2.1.17 (Muramidase)
SB  - IM
MH  - Anaphylaxis/immunology
MH  - Animals
MH  - *Antibody Formation
MH  - Arthus Reaction/immunology
MH  - Carboxylic Acids/immunology
MH  - *Cross Reactions
MH  - Epitopes
MH  - Female
MH  - Hypersensitivity, Delayed/immunology
MH  - *Immunity, Cellular
MH  - Immunoglobulin E/analysis
MH  - Immunoglobulin G/analysis
MH  - Immunologic Memory
MH  - Mice
MH  - Muramidase/*immunology
EDAT- 1975/02/01 00:00
MHDA- 1975/02/01 00:01
CRDT- 1975/02/01 00:00
PHST- 1975/02/01 00:00 [pubmed]
PHST- 1975/02/01 00:01 [medline]
PHST- 1975/02/01 00:00 [entrez]
AID - 10.7883/yoken1952.28.23 [doi]
PST - ppublish
SO  - Jpn J Med Sci Biol. 1975 Feb;28(1):23-35. doi: 10.7883/yoken1952.28.23.