PMID- 521057
OWN - NLM
STAT- MEDLINE
DCOM- 19800317
LR  - 20220408
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 38
IP  - 4
DP  - 1979 Dec
TI  - Activation of mouse complement by different classes of mouse antibody.
PG  - 687-95
AB  - The capacity of mouse IgM, IgGl, IgG2 and IgA anti-dinitrophenyl (DNP) antibodies 
      to activate mouse or guinea-pig complement was studied, using a sensitive 
      haemolytic assay and two-dimensional immunoelectrophoresis to detect cleavage of 
      mouse C3. Three monoclonal IgM antibodies, and a heterogeneous IgM fraction, 
      lysed trinitrophenylated erythrocytes in the presence of guinea-pig C, but failed 
      to produce lysis in the presence of mouse C. and only activated mouse C3 very 
      inefficiently. A monoclonal IgGl antibody did not produce haemolysis in the 
      presence of guinea-pig or mouse C, but cleaved mouse C3 via the alternative 
      pathway. Two IgA myeloma proteins (M315 and M460) had similar properties. A 
      heterogeneous IgG2 antibody fraction produced haemolysis in the presence of both 
      mouse and guinea-pig C, and was shown to activate both the classical and 
      alternative pathways of mouse C.
FAU - Klaus, G G
AU  - Klaus GG
FAU - Pepys, M B
AU  - Pepys MB
FAU - Kitajima, K
AU  - Kitajima K
FAU - Askonas, B A
AU  - Askonas BA
LA  - eng
PT  - Journal Article
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Complement C3)
RN  - 0 (Dinitrobenzenes)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Immunoglobulins)
RN  - 0 (Trinitrobenzenes)
RN  - 9007-36-7 (Complement System Proteins)
RN  - 9013-72-3 (Hemocyanins)
SB  - IM
MH  - Animals
MH  - Complement C3/*immunology
MH  - Complement Fixation Tests
MH  - Complement System Proteins/*immunology
MH  - Dinitrobenzenes/immunology
MH  - Erythrocytes/immunology
MH  - Guinea Pigs
MH  - Hemocyanins/immunology
MH  - Hemolysis
MH  - Immunoglobulin A
MH  - Immunoglobulin G
MH  - Immunoglobulin M
MH  - Immunoglobulins/*classification
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Rabbits
MH  - Sheep
MH  - Trinitrobenzenes/immunology
PMC - PMC1457877
EDAT- 1979/12/01 00:00
MHDA- 1979/12/01 00:01
PMCR- 1980/12/01
CRDT- 1979/12/01 00:00
PHST- 1979/12/01 00:00 [pubmed]
PHST- 1979/12/01 00:01 [medline]
PHST- 1979/12/01 00:00 [entrez]
PHST- 1980/12/01 00:00 [pmc-release]
PST - ppublish
SO  - Immunology. 1979 Dec;38(4):687-95.