PMID- 521313
OWN - NLM
STAT- MEDLINE
DCOM- 19800327
LR  - 20190827
IS  - 0301-5564 (Print)
IS  - 0301-5564 (Linking)
VI  - 64
IP  - 1
DP  - 1979 Nov
TI  - Microphotometric studies on intraacinar enzyme distribution in rat liver.
PG  - 23-33
AB  - Intraacinar distribution of succinate dehydrogenase (SDH), malate dehydrogenase 
      (MDH), NADP-dependent isocitrate dehydrogenase (IDH), glutamate dehydrogenase 
      (GluDH), lactate dehydrogenase (LDH) and NADH-tetrazolium dehydrogenase (TR) was 
      studied in rat liver cryostat sections by multipositional microphotometric 
      activity determinations. By statistical evaluation, activity of individual 
      enzymes could be related to the acinar topography. Activity was evaluated with 
      regard to distance of measuring position either from afferent (portal) or 
      efferent (hepatic) vessels. Two independent distribution curves were obtained for 
      each enzyme. Acinar distribution of all the enzymes studied followed sigmoid 
      courses with maximal activity of SDH, MDH and LDH in zone 1 ("periportal") and 
      GluDH, IDH, TR in zone 3 ("pericentral"). For all enzymes, maximum activity 
      gradients were confined to zone 2 of the acinus. Data were also evaluated as 
      ratios of activities in zone 1 and zone 3. The following ratios zone 1/zone 3 
      were obtained: SDH = 1.9, MDH = 1.7, IDH = 0.5, GluDH = 0.5, LDH = 1.3 and TR = 
      0.6.
FAU - Wimmer, M
AU  - Wimmer M
FAU - Pette, D
AU  - Pette D
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Histochemistry
JT  - Histochemistry
JID - 0411300
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.1.1.37 (Malate Dehydrogenase)
RN  - EC 1.1.1.41 (Isocitrate Dehydrogenase)
RN  - EC 1.3.99.1 (Succinate Dehydrogenase)
RN  - EC 1.4.1.2 (Glutamate Dehydrogenase)
RN  - EC 1.6.- (NADH Tetrazolium Reductase)
SB  - IM
MH  - Animals
MH  - Glutamate Dehydrogenase/analysis
MH  - *Histocytochemistry
MH  - Isocitrate Dehydrogenase/analysis
MH  - L-Lactate Dehydrogenase/analysis
MH  - Liver/*enzymology
MH  - Malate Dehydrogenase/analysis
MH  - Male
MH  - NADH Tetrazolium Reductase/analysis
MH  - Photomicrography/*methods
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Succinate Dehydrogenase/analysis
EDAT- 1979/11/01 00:00
MHDA- 1979/11/01 00:01
CRDT- 1979/11/01 00:00
PHST- 1979/11/01 00:00 [pubmed]
PHST- 1979/11/01 00:01 [medline]
PHST- 1979/11/01 00:00 [entrez]
AID - 10.1007/BF00493352 [doi]
PST - ppublish
SO  - Histochemistry. 1979 Nov;64(1):23-33. doi: 10.1007/BF00493352.