PMID- 52586
OWN - NLM
STAT- MEDLINE
DCOM- 19760126
LR  - 20190918
IS  - 0018-7348 (Print)
IS  - 0018-7348 (Linking)
VI  - 30
IP  - 1
DP  - 1975 Oct 20
TI  - Sequential Q- and Acridine orange-marker technique.
PG  - 83-90
AB  - A standardized Q- and acridine orange (AO)-fluorescence dual marker technique was 
      described. It involved preservation of unstained chromosome slides in a vacuum 
      desiccator up to 18 months, Q-staining, destaining, and treatment in Hanks' 
      solution, pH 5.1, at 85 degrees C for 13 min, and acridine orange staining. 
      Q-markers were found at the paracentromeric regions of chromosomes 3 and 4, the 
      short arms and the satellites of the acrocentric chromosomes, while AO-marker 
      spots were on the satellite-stalks of the acrocentrics. The advantage of the dual 
      marker technique was illustrated by the determination of the origin of trisomy 22 
      in a spontaneous abortus.
FAU - Niikawa, N
AU  - Niikawa N
FAU - Kajii, T
AU  - Kajii T
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Humangenetik
JT  - Humangenetik
JID - 7607154
RN  - 0 (Acridines)
RN  - 4213-45-0 (Quinacrine Mustard)
SB  - IM
MH  - Abortion, Spontaneous/genetics
MH  - Acridines
MH  - Chromosome Aberrations
MH  - Chromosomes/*analysis
MH  - Chromosomes, Human, 13-15
MH  - Chromosomes, Human, 21-22 and Y
MH  - Female
MH  - Humans
MH  - Male
MH  - Pregnancy
MH  - Quinacrine Mustard
MH  - Staining and Labeling
MH  - Trisomy
EDAT- 1975/10/20 00:00
MHDA- 1975/10/20 00:01
CRDT- 1975/10/20 00:00
PHST- 1975/10/20 00:00 [pubmed]
PHST- 1975/10/20 00:01 [medline]
PHST- 1975/10/20 00:00 [entrez]
AID - 10.1007/BF00273636 [doi]
PST - ppublish
SO  - Humangenetik. 1975 Oct 20;30(1):83-90. doi: 10.1007/BF00273636.