PMID- 52618 OWN - NLM STAT- MEDLINE DCOM- 19760202 LR - 20181113 IS - 0019-2805 (Print) IS - 1365-2567 (Electronic) IS - 0019-2805 (Linking) VI - 29 IP - 4 DP - 1975 Oct TI - Cell co-operation and hapten--carrier complexes. PG - 675-85 AB - The co-operation of spleen cells of carrier- and hapten--carrier-primed mice in antibody formation against the hapten part of complexes was studied in 550 rad whole body irradiated mice. Hapten--carrier complexes were prepared with the 2,4-dinitrophenyl group (DNP) as a hapten and heterologous bovine serum albumin (BSA) and isologous mouse immunoglobulin (MIg) as carriers. Priming of donor mice with carrier alone did not prepare for a secondary (IgG) response in the recipients of hapten--carrier. Priming of donors and challenge of recipients with the same hapten--carrier complex resulted in high IgG responses. Whereas donor and recipient immunization with complexes differing in the carrier did not give a secondary response, addition of cells of donors immunized with the carrier of the complex used for challenge, resulted in a secondary response. This was only possible when at least one of the complexes had an intermediate hapten:carrier ratio. Only an IgM or a low IgG response was obtained if both complexes had a high hapten:carrier ratio. Three determinants, namely hapten and carrier groups and new antigenic determinant (NAD), are suggested for antibody formation against hapten--protein complexes. In vivo treatment of donor cells with anti-thymocyte serum (ATS) or anti-plasma cell serum (APCS) and complement (C) suggested that: (1) T-cell epitopes are present on the carrier; (2) DNP groups are B-cell epitopes; (3) NAD and possibly DNP are T-cell epitopes; (4) synergism exists in the collaborative antibody response of B cells recongnizing DNP, T cell recognizing carrier and T cells recognizing NAD. Mitomycin treatment of donor cells was used to test whether cell division was mandatory. While the B cells were sensitive to mitomycin treatment, no effect of this drug was found on the helper activity of T cells. FAU - Snippe, H AU - Snippe H FAU - Kamp, E AU - Kamp E LA - eng PT - Journal Article PL - England TA - Immunology JT - Immunology JID - 0374672 RN - 0 (Antilymphocyte Serum) RN - 0 (Carrier Proteins) RN - 0 (Epitopes) RN - 0 (Haptens) RN - 0 (Immunoglobulin G) RN - 0 (Immunoglobulin M) RN - 0 (Immunoglobulins) RN - 0 (Mitomycins) RN - 0 (Nitrobenzenes) RN - 27432CM55Q (Serum Albumin, Bovine) RN - 9007-36-7 (Complement System Proteins) SB - IM MH - Animals MH - *Antibody Formation MH - Antibody-Producing Cells/*immunology MH - Antilymphocyte Serum MH - B-Lymphocytes/immunology MH - *Carrier Proteins MH - Complement System Proteins MH - Epitopes MH - *Haptens MH - Hemolytic Plaque Technique MH - Immunization, Passive MH - Immunization, Secondary MH - Immunoglobulin G MH - Immunoglobulin M MH - Immunoglobulins MH - Male MH - Mice MH - Mice, Inbred BALB C MH - Mitomycins/pharmacology MH - Mitosis/drug effects MH - Nitrobenzenes/immunology MH - Serum Albumin, Bovine MH - Spleen/immunology MH - T-Lymphocytes/immunology PMC - PMC1446068 EDAT- 1975/10/01 00:00 MHDA- 1975/10/01 00:01 PMCR- 1976/10/01 CRDT- 1975/10/01 00:00 PHST- 1975/10/01 00:00 [pubmed] PHST- 1975/10/01 00:01 [medline] PHST- 1975/10/01 00:00 [entrez] PHST- 1976/10/01 00:00 [pmc-release] PST - ppublish SO - Immunology. 1975 Oct;29(4):675-85.