PMID- 526428
OWN - NLM
STAT- MEDLINE
DCOM- 19800425
LR  - 20190515
IS  - 0007-0920 (Print)
IS  - 1532-1827 (Electronic)
IS  - 0007-0920 (Linking)
VI  - 40
IP  - 6
DP  - 1979 Dec
TI  - Cell-cycle distribution of urothelial tumour cells as measured by flow cytometry.
PG  - 872-7
AB  - The fraction of cells in S + G2 + mitosis from 54 urothelial tumours was 
      calculated by flow cytometry after acridine orange (AO) staining of cells 
      obtained by bladder irrigation or biopsy. Fluorescence signals emitted by the 
      AO-stained DNA and RNA of each cell were separated optically and measured for 
      5,000 cells per specimen. The patients were classified by the histology of their 
      tumours and clinical data into 5 diagnostic categories: NED (no evidence of 
      disease, but history of bladder tumour), 3; papilloma, 8; non-invasive papillary 
      carcinoma, 8; carcinoma in situ, 17 and invasive carcinoma, 18. The fraction of 
      cells with DNA values in S + G2 + M of the cell cycle varied between 7 and 57% of 
      the total, with a wide range within each diagnostic category, but no 
      statistically significant differences between the groups. The proportion of cells 
      in S + G2 + M from an individual tumour was not correlated with histologic grade 
      or clinical behaviour. The possibility that some tumour cells with DNA values 
      above G1 level are quiescent cells arrested at S or G2 is discussed.
FAU - Collste, L G
AU  - Collste LG
FAU - Darzynkiewicz, Z
AU  - Darzynkiewicz Z
FAU - Traganos, F
AU  - Traganos F
FAU - Sharpless, T K
AU  - Sharpless TK
FAU - Devonec, M
AU  - Devonec M
FAU - Claps, M L
AU  - Claps ML
FAU - Whitmore, W F Jr
AU  - Whitmore WF Jr
FAU - Melamed, M R
AU  - Melamed MR
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Br J Cancer
JT  - British journal of cancer
JID - 0370635
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Cell Count
MH  - Cell Cycle
MH  - DNA, Neoplasm/biosynthesis
MH  - Humans
MH  - Urinary Bladder Neoplasms/*pathology
PMC - PMC2010151
EDAT- 1979/12/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1979/12/01 00:00
PHST- 1979/12/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1979/12/01 00:00 [entrez]
AID - 10.1038/bjc.1979.279 [doi]
PST - ppublish
SO  - Br J Cancer. 1979 Dec;40(6):872-7. doi: 10.1038/bjc.1979.279.