PMID- 56389
OWN - NLM
STAT- MEDLINE
DCOM- 19760525
LR  - 20170214
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 24
IP  - 1
DP  - 1976 Jan
TI  - Quantitative fluorescence spectrophotometry of acridine orange-stained unfixed 
      cells. Potential for automated detection of human uterine cancer.
PG  - 315-21
AB  - After staining with acridine orange (AO), the nuclei of unfixed cells from the 
      human female genital tract exhibited the same fluorescence behavior previously 
      observed for human and murine leukocytes and mouse ascites tumor cells. With 
      staining conditions chosen to assure saturation of the green-fluorescing 
      AO-nucleic acid complex in normal cells, corrected fluorescence emission spectra 
      were recorded from the entire nucleus of 341 cells taken from 32 normal and 28 
      abnormal patients. Intensity of the recorded spectra was expressed in phosphor 
      particle units, a fixed arbitrary unit of fluorescence intensity, to display 
      intensity differences among the spectra from the various cell types. In all 
      abnormal samples, one or more cells were found with 530-nm nuclear fluorescence 
      intensity considerably greater than the maximum intensity recorded from normal 
      cells. Determination of the adequacy of 530-nm nuclear fluorescence intensity as 
      a criterion for cancer detection requires additional investigation. Additional 
      criteria, if needed, may be supplied by the metachromasy of AO-stained unfixed 
      cells.
FAU - Golden, J F
AU  - Golden JF
FAU - West, S S
AU  - West SS
FAU - Echols, C K
AU  - Echols CK
FAU - Shingleton, H M
AU  - Shingleton HM
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (Acridines)
SB  - IM
MH  - Acridines
MH  - Autoanalysis
MH  - Cervix Uteri/pathology
MH  - Cytodiagnosis/methods
MH  - Female
MH  - Humans
MH  - Microscopy, Fluorescence/methods
MH  - Spectrometry, Fluorescence/methods
MH  - Staining and Labeling
MH  - Uterine Neoplasms/*diagnosis/pathology
EDAT- 1976/01/01 00:00
MHDA- 1976/01/01 00:01
CRDT- 1976/01/01 00:00
PHST- 1976/01/01 00:00 [pubmed]
PHST- 1976/01/01 00:01 [medline]
PHST- 1976/01/01 00:00 [entrez]
AID - 10.1177/24.1.56389 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 1976 Jan;24(1):315-21. doi: 10.1177/24.1.56389.