PMID- 573271
OWN - NLM
STAT- MEDLINE
DCOM- 19791121
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 82
IP  - 1
DP  - 1979 Jul
TI  - Luteinizing hormone-accelerated redistribution of lysosome-like organelles 
      preceding dissolution of the nuclear envelope in rat oocytes maturing in vitro.
PG  - 264-77
AB  - Maturation of the mammalian oocyte is characterized in part by dissolution of the 
      nuclear envelope, or germinal vesicle breakdown (GVB). By fluorescence microscopy 
      after vital uptake of acridine orange (AO), redistribution and perinuclear 
      accumulation of organelles corresponding to lysosomes occur before GVB in rat 
      oocytes undergoing meiotic maturation in vitro. In follicle-enclosed oocytes 
      explanted during the preovulatory gonadotropin surge (GS) and individually 
      cultured as such in chemically defined medium at approximately 22 degrees C, 
      lysosomes aggregated into disperse clusters after 30 min; by 60 min, perinuclear 
      concentration of lysosomes and their essential disappearance from the cortical 
      ooplasm were observed. GVB occurred within 120 min. In contrast, 
      follicle-enclosed oocytes explanted before the GS displayed a generally 
      homogeneous distribution of lysosomes and intact GV for up to 5 h in culture. In 
      oocytes aspirated from follicles before the GS, partially denuded of granulosa 
      cells, and cultivated without added hormone, most lysosomes concentrated around 
      the GV within 60 min, with GVB occurring generally by 120 min. Luteinizing 
      hormone (LH) added in vitro to the isolated preparation at 3 or 30 x 10(-8) M 
      sharply accelerated these events. The effects of LH, not seen with 1.5 x 10(-8) M 
      hormone, were blocked by anti-LH IgG. Up to 60 x 10(-8) M follicle-stimulating 
      hormone or 80 x 10(-8) M prolactin were ineffective in accelerating lysosome 
      redistribution or GVB. After GVB, lysosomes became once again uniformly dispersed 
      and unresponsive, even to 60 x 10(-8) M added LH, a finding consistent with 
      tachyphylaxis of target cells by independent criteria. The present data, all 
      statistically significant at P less than 0.05, demonstrate that mobilization of 
      lysosomes before GVB is a specific response to factors that promote resumption of 
      meiotic maturation of rat oocytes.
FAU - Ezzell, R M
AU  - Ezzell RM
FAU - Szego, C M
AU  - Szego CM
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Gonadotropins)
RN  - 9002-62-4 (Prolactin)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Diestrus
MH  - Female
MH  - Follicle Stimulating Hormone/pharmacology
MH  - Gonadotropins/physiology
MH  - Luteinizing Hormone/*pharmacology
MH  - Lysosomes/*ultrastructure
MH  - Nuclear Envelope/ultrastructure
MH  - Oocytes/*growth & development/ultrastructure
MH  - Ovum/*growth & development
MH  - Pregnancy
MH  - Proestrus
MH  - Prolactin/pharmacology
MH  - Rats
PMC - PMC2110411
EDAT- 1979/07/01 00:00
MHDA- 1979/07/01 00:01
PMCR- 1980/01/01
CRDT- 1979/07/01 00:00
PHST- 1979/07/01 00:00 [pubmed]
PHST- 1979/07/01 00:01 [medline]
PHST- 1979/07/01 00:00 [entrez]
PHST- 1980/01/01 00:00 [pmc-release]
AID - 80006802 [pii]
AID - 10.1083/jcb.82.1.264 [doi]
PST - ppublish
SO  - J Cell Biol. 1979 Jul;82(1):264-77. doi: 10.1083/jcb.82.1.264.