PMID- 6036523
OWN - NLM
STAT- MEDLINE
DCOM- 19671019
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 33
IP  - 3
DP  - 1967 Jun
TI  - Thermal depolarization of fluorescence from polytene chromosomes stained with 
      acridine orange.
PG  - 597-604
AB  - The degree of polarization of fluorescence from stretched Chironomus thummi 
      polytene chromosomes, stained with low concentrations of acridine orange (AO), 
      decreases with increasing temperature. The "half temperature" of this decrease 
      (T((1/2)R)) is lower than the expected DNA thermal denaturation temperature 
      (T(m)) by about 20 degrees C. T((1/2)R) is lowered as histone is removed from 
      chromosomes. Balbiani ring regions of the fourth chromosome have T((1/2)R)'s much 
      lower than other regions, and nearly as low as chromosomes which had been 
      extensively pretreated with trypsin to remove histone and other proteins. 
      Measurements of the thermal change in the rotational diffusion rate of AO in 
      solution with DNA indicate that the temperature at which the DNA-AO bonding 
      changes from a "rigid" to a "loose" mode varies with the GC percentage of the 
      DNA, and in the same fashion as T(m), although 20 degrees C lower.
FAU - MacInnes, J W
AU  - MacInnes JW
FAU - Uretz, R B
AU  - Uretz RB
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Acridines)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - *Acridines
MH  - Animals
MH  - Chemical Phenomena
MH  - Chemistry, Physical
MH  - *Chromosomes
MH  - DNA
MH  - Diptera
MH  - *Fluorescence
MH  - Microscopy, Fluorescence
MH  - Salivary Glands
MH  - *Temperature
PMC - PMC2107200
EDAT- 1967/06/01 00:00
MHDA- 1967/06/01 00:01
PMCR- 1967/12/01
CRDT- 1967/06/01 00:00
PHST- 1967/06/01 00:00 [pubmed]
PHST- 1967/06/01 00:01 [medline]
PHST- 1967/06/01 00:00 [entrez]
PHST- 1967/12/01 00:00 [pmc-release]
AID - 10.1083/jcb.33.3.597 [doi]
PST - ppublish
SO  - J Cell Biol. 1967 Jun;33(3):597-604. doi: 10.1083/jcb.33.3.597.