PMID- 6087266
OWN - NLM
STAT- MEDLINE
DCOM- 19840906
LR  - 20190726
IS  - 0031-6768 (Print)
IS  - 0031-6768 (Linking)
VI  - 400
IP  - 4
DP  - 1984 Apr
TI  - Effects of chemical modification of amino and sulfhydryl groups on the 
      voltage-clamped frog node of Ranvier.
PG  - 403-8
AB  - Several reagents that react with sulfhydryl and amino groups were applied to 
      voltage-clamped single nerve fibres of the frog. The fibres were exposed to 
      comparable amounts of the chemical reagents for relatively short times. 
      3-(p-Hydroxyphenyl)propionic acid N-hydroxysuccinimide ester (HPPS), a substance 
      which preferentially modifies amino groups, irreversibly reduced the size of the 
      sodium and potassium current. The effect of HPPS on the Na current could be 
      removed only partially by hyperpolarizing prepulses. N-ethylmaleimide (NEM), a 
      reagent that preferentially reacts with sulfhydryl groups produced a small 
      decrease of the sodium current which was removed almost completely by 
      hyperpolarizing prepulses. NEM and HPPS shifted the voltage dependence of sodium 
      inactivation, h infinity (E), to more negative values of membrane potential, but 
      had little effect on the time course of sodium activation and inactivation. 
      Pretreatment of a fibre with NEM did not prevent the action of HPPS; however, 
      pretreatment of a fibre with HPPS decreased considerably the shift of the h 
      infinity (E) curve caused by NEM. Our results suggest that modification of 
      membrane bound amino groups affects the size of the ionic currents and the 
      inactivation process. Although reagents that react with sulfhydryl groups were 
      found to affect channel function, no definite evidence for the presence or 
      absence of a functionally important sulfhydryl group on sodium channels has been 
      obtained.
FAU - Rack, M
AU  - Rack M
FAU - Hu, S L
AU  - Hu SL
FAU - Rubly, N
AU  - Rubly N
FAU - Waschow, C
AU  - Waschow C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Pflugers Arch
JT  - Pflugers Archiv : European journal of physiology
JID - 0154720
RN  - 0 (Amines)
RN  - 0 (Disulfides)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Ion Channels)
RN  - 0 (Pyridines)
RN  - 0 (Succinimides)
RN  - 0 (Sulfhydryl Compounds)
RN  - 2645-22-9 (4,4'-dipyridyl disulfide)
RN  - 2OHG8V08NL (N-succinimidyl 3-(2-pyridyldithio)propionate)
RN  - 34071-95-9 (N-succinimidyl-3-(4-hydroxyphenyl)propionate)
RN  - O3C74ACM9V (Ethylmaleimide)
SB  - IM
MH  - *Amines
MH  - Animals
MH  - *Disulfides
MH  - Electrophysiology
MH  - Ethylmaleimide/pharmacology
MH  - Indicators and Reagents
MH  - Ion Channels/drug effects
MH  - Pyridines/pharmacology
MH  - Rana esculenta
MH  - Ranvier's Nodes/*drug effects/physiology
MH  - Succinimides/pharmacology
MH  - *Sulfhydryl Compounds
EDAT- 1984/04/01 00:00
MHDA- 1984/04/01 00:01
CRDT- 1984/04/01 00:00
PHST- 1984/04/01 00:00 [pubmed]
PHST- 1984/04/01 00:01 [medline]
PHST- 1984/04/01 00:00 [entrez]
AID - 10.1007/BF00587540 [doi]
PST - ppublish
SO  - Pflugers Arch. 1984 Apr;400(4):403-8. doi: 10.1007/BF00587540.