PMID- 6090130
OWN - NLM
STAT- MEDLINE
DCOM- 19841113
LR  - 20220511
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 3
IP  - 8
DP  - 1984 Aug
TI  - Modulation of enhancer activity by the hormone responsive regulatory element from 
      mouse mammary tumor virus.
PG  - 1891-9
AB  - Addition of the transcriptional enhancers present in the U3 region of the Harvey 
      murine sarcoma virus (HaMuSV) long terminal repeat (LTR) to recombinant chimeras 
      in which the HaMuSV transforming gene (Ha-v-ras) is expressed from the mouse 
      mammary tumor virus (MMTV) promoter increases the ability of the MMTV v-ras 
      chimeras to transform mouse fibroblasts in culture 50- to 100-fold. Significant 
      stimulation of transfection efficiency occurs only when glucocorticoids are 
      present in the culture medium. Glucocorticoids also elevate the steady-state 
      concentration of MMTV-initiated v-ras mRNA in cell lines isolated from these 
      transfections, and MMTV-v-ras fusion transcripts are initiated at the normal MMTV 
      cap site; potential cryptic initiation events associated with the enhancer could 
      not be detected. The ability of the enhancer to increase the transcriptional 
      activity of the MMTV promoter was also studied in acute transfection assays where 
      expression of the chloramphenical acetyl transferase (CAT) gene is driven by the 
      MMTV promoter. In this system the strong positive effect on MMTV transcription is 
      again obtained only when the cells are hormone treated. These experiments 
      indicate that the hormone-regulatory region is capable of modulating the function 
      of an exogenously introduced enhancer element.
FAU - Ostrowski, M C
AU  - Ostrowski MC
FAU - Huang, A L
AU  - Huang AL
FAU - Kessel, M
AU  - Kessel M
FAU - Wolford, R G
AU  - Wolford RG
FAU - Hager, G L
AU  - Hager GL
LA  - eng
PT  - Journal Article
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (RNA Caps)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Receptors, Steroid)
RN  - 0 (dexamethasone receptor)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 2.3.1.- (Acetyltransferases)
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
SB  - IM
MH  - Acetyltransferases/biosynthesis
MH  - Animals
MH  - Cell Transformation, Viral
MH  - Chloramphenicol O-Acetyltransferase
MH  - Dexamethasone/*pharmacology
MH  - Gene Expression Regulation/*drug effects
MH  - Genetic Vectors
MH  - Harvey murine sarcoma virus/*genetics
MH  - Mice
MH  - Oncogenes
MH  - Operon
MH  - RNA Caps
MH  - RNA, Messenger/genetics
MH  - RNA, Viral/genetics
MH  - Receptors, Glucocorticoid/*physiology
MH  - Receptors, Steroid/*physiology
MH  - *Repetitive Sequences, Nucleic Acid
MH  - Sarcoma Viruses, Murine/*genetics
MH  - Transcription, Genetic/*drug effects
PMC - PMC557614
EDAT- 1984/08/01 00:00
MHDA- 1984/08/01 00:01
PMCR- 1985/08/01
CRDT- 1984/08/01 00:00
PHST- 1984/08/01 00:00 [pubmed]
PHST- 1984/08/01 00:01 [medline]
PHST- 1984/08/01 00:00 [entrez]
PHST- 1985/08/01 00:00 [pmc-release]
AID - 10.1002/j.1460-2075.1984.tb02064.x [doi]
PST - ppublish
SO  - EMBO J. 1984 Aug;3(8):1891-9. doi: 10.1002/j.1460-2075.1984.tb02064.x.