PMID- 6091899
OWN - NLM
STAT- MEDLINE
DCOM- 19841126
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 38
IP  - 3
DP  - 1984 Oct
TI  - Hormonal regulation of the Rous sarcoma virus src gene via a heterologous 
      promoter defines a threshold dose for cellular transformation.
PG  - 757-65
AB  - We have derived rat cell lines producing different and regulatable amounts of 
      pp60v-src by introducing the src gene of Rous sarcoma virus (RSV) under the 
      control of the glucocorticoid-responsive transcriptional promoter from the mouse 
      mammary tumor virus (MMTV). We find that the cellular phenotype is strictly 
      dependent upon the dose of pp60v-src with a distinct threshold for changes 
      indicative of neoplastic potential. Cells with low constitutive levels of 
      pp60v-src are not phenotypically distinguishable from cells without v-src, but as 
      little as a 4-fold increment in pp60v-src produces morphological transformation 
      and anchorage-independent growth. These properties of the transformed state are 
      achieved at levels of pp60v-src far below levels found in an RSV-transformed cell 
      line, without detectable increase in phosphorylation of the major cellular target 
      for tyrosine phosphorylation.
FAU - Jakobovits, E B
AU  - Jakobovits EB
FAU - Majors, J E
AU  - Majors JE
FAU - Varmus, H E
AU  - Varmus HE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Viral Proteins)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.10.2 (Oncogene Protein pp60(v-src))
RN  - EC 3.1.21.- (DNA Restriction Enzymes)
SB  - IM
MH  - Animals
MH  - Avian Sarcoma Viruses/enzymology/*genetics
MH  - Cell Line
MH  - *Cell Transformation, Neoplastic
MH  - DNA Restriction Enzymes
MH  - Dexamethasone/*pharmacology
MH  - Genes/*drug effects
MH  - Genes, Viral/*drug effects
MH  - Genetic Vectors
MH  - Mammary Tumor Virus, Mouse/genetics
MH  - Mice
MH  - Oncogene Protein pp60(v-src)
MH  - Phosphorylation
MH  - Plasmids
MH  - Protein Kinases/*genetics
MH  - Rats
MH  - Transcription, Genetic/*drug effects
MH  - Transfection
MH  - Viral Proteins/*genetics/metabolism
EDAT- 1984/10/01 00:00
MHDA- 1984/10/01 00:01
CRDT- 1984/10/01 00:00
PHST- 1984/10/01 00:00 [pubmed]
PHST- 1984/10/01 00:01 [medline]
PHST- 1984/10/01 00:00 [entrez]
AID - 0092-8674(84)90271-X [pii]
AID - 10.1016/0092-8674(84)90271-x [doi]
PST - ppublish
SO  - Cell. 1984 Oct;38(3):757-65. doi: 10.1016/0092-8674(84)90271-x.