PMID- 6091914
OWN - NLM
STAT- MEDLINE
DCOM- 19841212
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 39
IP  - 1
DP  - 1984 Nov
TI  - Structure and nucleotide sequence of the putative mammary oncogene int-1; 
      proviral insertions leave the protein-encoding domain intact.
PG  - 233-40
AB  - Many mammary tumors induced by mouse mammary tumor virus (MMTV) contain a 
      provirus in the same region of the host-cell genome, leading to expression of a 
      putative cellular oncogene called int-1. Here we present the structure and 
      nucleotide sequence of int-1. We have established several proviral insertion 
      sites exactly by nuclease S1 analysis or by molecular cloning and DNA sequencing. 
      The protein-encoding domain of int-1 is distributed over four exons. At the 5' 
      end of the gene two overlapping exons were detected, one of which is preceded by 
      a TATA box. The deduced int-1-encoded protein has 370 amino acids, with a 
      preponderance of hydrophobic residues at the NH2 terminus. Proviruses are found 
      at both sides of the gene, usually oriented away from the gene. Downstream 
      integrations occur frequently in the long 3' untranslated region of the last 
      exon. One upstream provirus is inserted in the 5' untranslated region and, unlike 
      the other upstream insertions, in the same orientation as the int-1 gene. 
      Proviral integrations always leave the protein-encoding domain intact, providing 
      further evidence that the int-1 protein contributes an essential step in mammary 
      tumorigenesis.
FAU - van Ooyen, A
AU  - van Ooyen A
FAU - Nusse, R
AU  - Nusse R
LA  - eng
SI  - GENBANK/K02593
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (DNA Transposable Elements)
RN  - EC 3.1.- (Endonucleases)
RN  - EC 3.1.21.- (DNA Restriction Enzymes)
RN  - EC 3.1.30.1 (Single-Strand Specific DNA and RNA Endonucleases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA Restriction Enzymes
MH  - *DNA Transposable Elements
MH  - Endonucleases
MH  - *Genes
MH  - *Genes, Viral
MH  - Mammary Neoplasms, Experimental/microbiology
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Mice
MH  - *Oncogenes
MH  - Single-Strand Specific DNA and RNA Endonucleases
MH  - Transcription, Genetic
EDAT- 1984/11/01 00:00
MHDA- 1984/11/01 00:01
CRDT- 1984/11/01 00:00
PHST- 1984/11/01 00:00 [pubmed]
PHST- 1984/11/01 00:01 [medline]
PHST- 1984/11/01 00:00 [entrez]
AID - 0092-8674(84)90209-5 [pii]
AID - 10.1016/0092-8674(84)90209-5 [doi]
PST - ppublish
SO  - Cell. 1984 Nov;39(1):233-40. doi: 10.1016/0092-8674(84)90209-5.