PMID- 6092699
OWN - NLM
STAT- MEDLINE
DCOM- 19841218
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 52
IP  - 3
DP  - 1984 Dec
TI  - Mouse mammary tumor virus-related sequences in mouse lymphocytes are inducible by 
      12-O-tetradecanoyl phorbol-13-acetate.
PG  - 1000-4
AB  - cDNA libraries from EL-4 cells treated with 12-O-tetradecanoyl phorbol-13-acetate 
      (TPA) were screened for TPA-inducible sequences by differential hybridization. 
      The most abundant inducible species was a sequence similar to that of mouse 
      mammary tumor virus (MMTV). Induction of the mRNA corresponding to the 
      MMTV-related sequences was already evident 30 min after TPA treatment, whereas 
      the maximum accumulation occurred after 20 h of exposure to TPA. TPA also 
      increased levels of MMTV-related RNA in the normal spleen cells of BALB/c and 
      C57BL/6 mice. The level of RNA expression corresponding to MMTV-related 
      sequences, however, was markedly elevated in EL-4 cells as compared with normal 
      spleen cells. Southern blots of EL-4 cell DNA showed that the MMTV-related 
      sequences were inserted into multiple locations of the EL-4 genome. Sequence 
      analysis revealed that the MMTV-related cDNA clones included a part of the env 
      gene and the right long terminal repeat of MMTV. However, the cDNA sequences were 
      substantially different from published MMTV proviral sequences, most notably 
      because of a contiguous deletion of 491 base pairs in the open reading frame 
      within the U3 region.
FAU - Kwon, B S
AU  - Kwon BS
FAU - Weissman, S M
AU  - Weissman SM
LA  - eng
SI  - GENBANK/M11024
GR  - CA 30938-03/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Phorbols)
RN  - 0 (RNA, Messenger)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Gene Expression Regulation/drug effects
MH  - *Genes, Viral
MH  - Lymphocytes/*physiology
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Phorbols/*pharmacology
MH  - RNA, Messenger/genetics
MH  - Spleen/physiology
MH  - Tetradecanoylphorbol Acetate/*pharmacology
MH  - Transcription, Genetic/drug effects
PMC - PMC254629
EDAT- 1984/12/01 00:00
MHDA- 1984/12/01 00:01
PMCR- 1984/12/01
CRDT- 1984/12/01 00:00
PHST- 1984/12/01 00:00 [pubmed]
PHST- 1984/12/01 00:01 [medline]
PHST- 1984/12/01 00:00 [entrez]
PHST- 1984/12/01 00:00 [pmc-release]
AID - 10.1128/JVI.52.3.1000-1004.1984 [doi]
PST - ppublish
SO  - J Virol. 1984 Dec;52(3):1000-4. doi: 10.1128/JVI.52.3.1000-1004.1984.