PMID- 6093009
OWN - NLM
STAT- MEDLINE
DCOM- 19841220
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 50
IP  - 1-3
DP  - 1984 Sep 7
TI  - Up-regulation of opiate receptors by opiate antagonists in neuroblastoma-glioma 
      cell culture: the possibility of interaction with guanosine triphosphate-binding 
      proteins.
PG  - 133-7
AB  - Neuroblastoma-glioma NG108-15 cells that were cultured for 48 h with the opiate 
      antagonist, naloxone, respond to the guanosine 5'-triphosphate (GTP) analogue 
      guanosine 5'-[beta, gamma-imido]-triphosphate (GMP-PNP) in the binding assay as 
      the control, non-treated, cells. This was observed when the guanyl nucleotide was 
      tested in the presence or absence of sodium chloride and also after subcellular 
      fractionation of the membranes on a sucrose gradient which separated between two 
      receptor-containing fractions. The findings suggest that the increase in delta 
      type enkephalin receptors in naloxone-treated NG108-15 cells does not reflect an 
      alteration in the interaction between the receptor and the adenylate 
      cyclase-GTP-binding protein system.
FAU - Barg, J
AU  - Barg J
FAU - Levy, R
AU  - Levy R
FAU - Simantov, R
AU  - Simantov R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Receptors, Opioid)
RN  - 0 (Receptors, Opioid, delta)
RN  - 34273-04-6 (Guanylyl Imidodiphosphate)
RN  - 36B82AMQ7N (Naloxone)
RN  - 58822-25-6 (Enkephalin, Leucine)
RN  - 63631-40-3 (Enkephalin, Leucine-2-Alanine)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Enkephalin, Leucine/analogs & derivatives/metabolism
MH  - Enkephalin, Leucine-2-Alanine
MH  - GTP-Binding Proteins/*metabolism
MH  - Glioma
MH  - Guanosine Triphosphate/pharmacology
MH  - Guanylyl Imidodiphosphate/pharmacology
MH  - Naloxone/*pharmacology
MH  - Neuroblastoma
MH  - Rats
MH  - Receptors, Opioid/*drug effects/metabolism
MH  - Receptors, Opioid, delta
EDAT- 1984/09/07 00:00
MHDA- 1984/09/07 00:01
CRDT- 1984/09/07 00:00
PHST- 1984/09/07 00:00 [pubmed]
PHST- 1984/09/07 00:01 [medline]
PHST- 1984/09/07 00:00 [entrez]
AID - 0304-3940(84)90475-0 [pii]
AID - 10.1016/0304-3940(84)90475-0 [doi]
PST - ppublish
SO  - Neurosci Lett. 1984 Sep 7;50(1-3):133-7. doi: 10.1016/0304-3940(84)90475-0.