PMID- 6094742
OWN - NLM
STAT- MEDLINE
DCOM- 19841227
LR  - 20191101
IS  - 0270-6474 (Print)
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 4
IP  - 11
DP  - 1984 Nov
TI  - Modification of guanine nucleotide-regulatory components in brain membranes. II. 
      Relationship of guanosine 5'-triphosphate effects on opiate receptor binding and 
      coupling receptors with adenylate cyclase.
PG  - 2764-71
AB  - Guanine nucleotides couple receptors to stimulate or inhibit adenylate cyclase as 
      well as regulate binding of neurotransmitters. To explore the relationship 
      between these different functions of guanosine 5'-triphosphate (GTP), rat brain 
      membranes were preincubated in 50 mM sodium acetate, pH 4.5, which increased GTP 
      regulation of 3H-opiate agonist binding. Assay of adenylate cyclase in the low 
      pH-pretreated membranes revealed no loss of basal activity but a dramatic loss in 
      fluoride- and guanylyl-5'-imidodiphosphate-stimulated activity, thus suggesting a 
      loss in stimulatory guanine nucleotide coupling function. Manganese stimulation, 
      which presumably occurs on the catalytic subunit of adenylate cyclase directly, 
      was not affected by low pH treatment. In striatum, dopamine-stimulated adenylate 
      cyclase was eliminated, but inhibition of adenylate cyclase by 
      D-Ala2-Met5-enkephalinamide (D-Ala enk) was increased by low pH treatment. The 
      effect of low pH on sodium fluoride-stimulated and D-Ala enk-inhibited adenylate 
      cyclase could be reversed by addition of either cis-vaccenic acid or 
      phosphatidylcholine to treated membranes, but the effect on GTP regulation of 
      binding was not reversed by lipid incorporation. These results suggest that 
      fundamental differences exist between membrane components which couple receptors 
      to adenylate cyclase and those that regulate neurotransmitter binding.
FAU - Childers, S R
AU  - Childers SR
FAU - LaRiviere, G
AU  - LaRiviere G
LA  - eng
GR  - DA-02094/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Membrane Lipids)
RN  - 0 (Oleic Acids)
RN  - 0 (Phosphatidylcholines)
RN  - 0 (Receptors, Opioid)
RN  - 34273-04-6 (Guanylyl Imidodiphosphate)
RN  - 506-17-2 (cis-vaccenic acid)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - 8ZYQ1474W7 (Sodium Fluoride)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
SB  - IM
MH  - Adenylyl Cyclases/*metabolism
MH  - Animals
MH  - Brain/drug effects/*enzymology
MH  - Cell Membrane/physiology
MH  - Corpus Striatum/enzymology
MH  - Guanosine Triphosphate/*pharmacology
MH  - Guanylyl Imidodiphosphate/pharmacology
MH  - Hydrogen-Ion Concentration
MH  - Male
MH  - Membrane Fluidity
MH  - Membrane Lipids/physiology
MH  - Oleic Acids/pharmacology
MH  - Phosphatidylcholines/pharmacology
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Receptors, Opioid/drug effects/*metabolism
MH  - Sodium Fluoride/pharmacology
MH  - Viscosity
PMC - PMC6564728
EDAT- 1984/11/01 00:00
MHDA- 1984/11/01 00:01
PMCR- 1985/05/01
CRDT- 1984/11/01 00:00
PHST- 1984/11/01 00:00 [pubmed]
PHST- 1984/11/01 00:01 [medline]
PHST- 1984/11/01 00:00 [entrez]
PHST- 1985/05/01 00:00 [pmc-release]
AID - 10.1523/JNEUROSCI.04-11-02764.1984 [doi]
PST - ppublish
SO  - J Neurosci. 1984 Nov;4(11):2764-71. doi: 10.1523/JNEUROSCI.04-11-02764.1984.