PMID- 6097815
OWN - NLM
STAT- MEDLINE
DCOM- 19850314
LR  - 20081121
IS  - 0026-8984 (Print)
IS  - 0026-8984 (Linking)
VI  - 18
IP  - 6
DP  - 1984 Nov-Dec
TI  - [Study of the secondary and tertiary structure of phage MS2 RNA using nucleases 
      and fluorescent dyes specific for secondary structure].
PG  - 1625-33
AB  - The binding of ethidium bromide (EtBr) and acridine orange (AO) to RNA in native 
      state or after hydrolysis by S1 and SV nucleases that specifically split 
      single-stranded and double-stranded segments was studied. Nuclease S1 hydrolysis 
      of RNA does not increase the number of EtBr strong binding sites, Tm and 
      hyperchromic effect being also unchanged. Hydrolysis by double-stranded segments 
      accessible to EtBr is followed by the diminishing of Tm and hyperchromism. A 
      supposition is put forward that the main role in stabilization of the RNA 
      tertiary structure is played by double-stranded segments arranged so that some of 
      them are hidden and do not interact with dyes. One of the possible models may be 
      parallel oriented intramolecular "hair-pins" forming compact "rod-like" 
      structures.
FAU - Borisova, O F
AU  - Borisova OF
FAU - Grechko, V V
AU  - Grechko VV
FAU - Aleshkina, L A
AU  - Aleshkina LA
FAU - Kuznetsova, N V
AU  - Kuznetsova NV
LA  - rus
PT  - English Abstract
PT  - Journal Article
TT  - Izuchenie vtorichnoi i tretichnoi struktury RNK faga MS2 s ispol'zovaniem nukleaz 
      i fluorestsiruiushchikh krasitelei, spetsifichnykh ko vtorichnoi strukture.
PL  - Russia (Federation)
TA  - Mol Biol (Mosk)
JT  - Molekuliarnaia biologiia
JID - 0105454
RN  - 0 (Fluorescent Dyes)
RN  - 0 (RNA, Double-Stranded)
RN  - 0 (RNA, Viral)
RN  - EC 3.1.- (Endonucleases)
RN  - EC 3.1.- (Endoribonucleases)
RN  - EC 3.1.26.- (cobra venom endonuclease)
RN  - EC 3.1.30.1 (Single-Strand Specific DNA and RNA Endonucleases)
SB  - IM
MH  - *Coliphages
MH  - *Endonucleases
MH  - *Endoribonucleases
MH  - *Fluorescent Dyes
MH  - Hydrolysis
MH  - *Nucleic Acid Conformation
MH  - RNA, Double-Stranded
MH  - *RNA, Viral
MH  - Single-Strand Specific DNA and RNA Endonucleases
EDAT- 1984/11/01 00:00
MHDA- 1984/11/01 00:01
CRDT- 1984/11/01 00:00
PHST- 1984/11/01 00:00 [pubmed]
PHST- 1984/11/01 00:01 [medline]
PHST- 1984/11/01 00:00 [entrez]
PST - ppublish
SO  - Mol Biol (Mosk). 1984 Nov-Dec;18(6):1625-33.