PMID- 6100310
OWN - NLM
STAT- MEDLINE
DCOM- 19850823
LR  - 20131121
IS  - 0021-7948 (Print)
IS  - 0021-7948 (Linking)
VI  - 79
IP  - 6
DP  - 1984
TI  - Angiotensin biosynthesis and concentrations in brain of normotensive and 
      hypertensive rats.
PG  - 471-80
AB  - We report here on the extraction and characterization of angiotensin I (ANG I) 
      and angiotensin II (ANG II) from the brain of rats. High pressure liquid 
      chromatography (HPLC) with different mobile phases combined with specific 
      radioimmunoassays (RIA) proved to be a powerful tool for peptide characterization 
      in biological samples; (Ile5)-ANG I, (Ile5)-ANG II and (Ile5)-ANG III could 
      clearly be identified in cerebrospinal fluid (CSF), incubated in vivo and in 
      vitro with renin, in total brain extracts, as well as in hypothalamus (HT), 
      medulla oblongata (MO), cerebellum (CER) and cortex (CO). Angiotensin cleaved 
      from CSF angiotensinogen and angiotensin extracted from brain showed retention 
      times identical to those of plasma angiotensin and synthetic standard peptides, 
      indicating that their amino acid sequence is probably identical. ANG I and ANG II 
      were highest in the HT and lowest in the CO. Following bilateral nephrectomy (NX) 
      both ANG I and ANG II persisted at control levels. Young 10 week old 
      spontaneously hypertensive rats (SHRSP) showed significantly lower ANG I and ANG 
      II concentrations in the HT compared with Wistar Kyoto rats (WKY). 
      Intracerebroventricular (i.c.v.) administration of the converting enzyme 
      inhibitor captopril caused a significant increase in ANG 1 in nephrectomized 
      SHRSP but not in WKY. These differences were not found in 40 week old SHRSP. The 
      data show that ANG I and ANG II are synthetized in the brain of rats. The lower 
      concentrations and the enhanced accumulation of ANG I after converting enzyme 
      blockade in nephrectomized young SHRSP indicate an increased turnover of 
      angiotensin in hypertensive rats.
FAU - Hermann, K
AU  - Hermann K
FAU - McDonald, W
AU  - McDonald W
FAU - Unger, T
AU  - Unger T
FAU - Lang, R E
AU  - Lang RE
FAU - Ganten, D
AU  - Ganten D
LA  - eng
PT  - Journal Article
PL  - France
TA  - J Physiol (Paris)
JT  - Journal de physiologie
JID - 9309350
RN  - 0 (Angiotensin-Converting Enzyme Inhibitors)
RN  - 0 (Angiotensins)
RN  - 0 (Peptide Fragments)
RN  - 11128-99-7 (Angiotensin II)
RN  - 12687-51-3 (Angiotensin III)
RN  - 9041-90-1 (Angiotensin I)
RN  - 9G64RSX1XD (Captopril)
SB  - IM
MH  - Angiotensin I/*biosynthesis/isolation & purification
MH  - Angiotensin II/*biosynthesis/isolation & purification
MH  - Angiotensin III/isolation & purification
MH  - Angiotensin-Converting Enzyme Inhibitors
MH  - Angiotensins/*biosynthesis/cerebrospinal fluid
MH  - Animals
MH  - Brain/*metabolism
MH  - Captopril/pharmacology
MH  - Chromatography, High Pressure Liquid
MH  - Female
MH  - Hypertension/*metabolism
MH  - Male
MH  - Nephrectomy
MH  - Peptide Fragments/isolation & purification
MH  - Rats
MH  - Rats, Inbred SHR
MH  - Rats, Inbred WKY
MH  - Tissue Distribution
EDAT- 1984/01/01 00:00
MHDA- 1984/01/01 00:01
CRDT- 1984/01/01 00:00
PHST- 1984/01/01 00:00 [pubmed]
PHST- 1984/01/01 00:01 [medline]
PHST- 1984/01/01 00:00 [entrez]
PST - ppublish
SO  - J Physiol (Paris). 1984;79(6):471-80.