PMID- 6105925
OWN - NLM
STAT- MEDLINE
DCOM- 19801124
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 21
IP  - 1
DP  - 1980 Aug
TI  - Isolation of glucocorticoid-unresponsive rat hepatoma cells by 
      fluorescence-activated cell sorting.
PG  - 47-56
AB  - We have used a mouse mammary tumor virus (MMTV)-infected rat hepatoma cell line 
      as a model system for studying glucocorticoid action. These cells induce tyrosine 
      aminotransferase and MMTV in response to the synthetic glucocorticoid, 
      dexamethasone. The major viral antigen, a glycoprotein of 52,000 daltons (gp52), 
      appears on the surface of infected cells in amounts which reflect the cytoplasmic 
      content of viral RNA. Using an anti-gp52 antiserum and a fluorescence-activated 
      cell sorter (FACS), we have selected variants which display low levels of pg52 in 
      the presence of the hormone. Multiple cycles of enrichment for cells that 
      fluoresce weakly in the presence of hormone have generated a population which 
      fails to produce a detectable increase in cell surface gp52 in response to 
      dexamethasone. This population of nonresponders and a number of independent 
      clones derived from this population were analyzed for their ability to induce 
      gp52 and TAT and for these presence of glucocorticoid receptors. All nonresponder 
      clones exhibited little or no induction of either glucocorticoid-inducible 
      marker. Two of the clones contained reduced levels of glucocrticoid receptor, 
      while the remainder of the clones showed no detectable specific hormone binding. 
      These results provide genetic evidence that a single class of glucocorticoid 
      receptors is involved in the induction of both MMTV and TAT in HTC cells.
FAU - Grove, J R
AU  - Grove JR
FAU - Dieckmann, B S
AU  - Dieckmann BS
FAU - Schroer, T A
AU  - Schroer TA
FAU - Ringold, G M
AU  - Ringold GM
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Receptors, Steroid)
RN  - 0 (Viral Proteins)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 2.6.1.5 (Tyrosine Transaminase)
SB  - IM
MH  - Animals
MH  - Carcinoma, Hepatocellular
MH  - Cell Line
MH  - *Cell Separation/methods
MH  - Dexamethasone/*pharmacology
MH  - Enzyme Induction
MH  - Liver Neoplasms
MH  - Mammary Tumor Virus, Mouse
MH  - Rats
MH  - Receptors, Glucocorticoid/*metabolism
MH  - Receptors, Steroid/*metabolism
MH  - Spectrometry, Fluorescence
MH  - Tyrosine Transaminase/*biosynthesis
MH  - Viral Proteins/*biosynthesis
EDAT- 1980/08/01 00:00
MHDA- 1980/08/01 00:01
CRDT- 1980/08/01 00:00
PHST- 1980/08/01 00:00 [pubmed]
PHST- 1980/08/01 00:01 [medline]
PHST- 1980/08/01 00:00 [entrez]
AID - 0092-8674(80)90113-0 [pii]
AID - 10.1016/0092-8674(80)90113-0 [doi]
PST - ppublish
SO  - Cell. 1980 Aug;21(1):47-56. doi: 10.1016/0092-8674(80)90113-0.