PMID- 6111556
OWN - NLM
STAT- MEDLINE
DCOM- 19810625
LR  - 20210526
IS  - 0021-9193 (Print)
IS  - 1098-5530 (Electronic)
IS  - 0021-9193 (Linking)
VI  - 146
IP  - 2
DP  - 1981 May
TI  - Response of guanosine 5'-triphosphate concentration to nutritional changes and 
      its significance for Bacillus subtilis sporulation.
PG  - 605-13
AB  - We have investigated the changes in the guanosine 5'-triphosphate (GTP) and 
      P-ribosyl-PP pools in stringent and relaxed strains of Bacillus subtilis under 
      conditions frequently used to initiate sporulation. After a shift-down from a 
      Casamino Acids-glutamate to a glutamate medium (Sterlini-Mandelstam shift-down), 
      the pools of adenosine 5'-triphosphate and P-ribosyl-PP increased in both 
      strains; in the stringent strain, ppGpp and pppGpp increased and GTP decreased 
      rapidly, whereas in the relaxed strain, ppGpp and pppGpp increased only slightly 
      and GTP decreased only slowly and less extensively. The stringent strain 
      sporulated well, whereas the relaxed strain sporulated late and poorly. Addition 
      of decoyinine, an inhibitor of guanosine 5'-monophosphate synthetase, caused a 
      further decrease of GTP and initiated good sporulation of the relaxed strain. 
      After a shift-down from a glucose-lactate to a lactate medium (Ramaley-Burden 
      shift-down) the pool of P-ribosyl-PP (and GTP) decreased in both strains, 
      indicating a shortage of purine precursors. This shift-down also caused a 
      stringent response which prevented the consumption of nucleotides, as shown by 
      the maintenance of adenosine 5'-triphosphate at a high concentration in the 
      stringent strain but not in the relaxed strain. After a delay, the relaxed 
      strain, in which GTP decreased as fast as in the stringent strain, sporulated 
      also as efficiently. In nutrient sporulation medium the stringent strain and, 
      less effectively, the relaxed strain accumulated ppGpp and pppGpp transiently 
      towards the end of exponential growth. Eventually, the P-ribosyl-PP pool 
      decreased drastically in both strains. In all cases the initiation of sporulation 
      was correlated with a significant decrease of GTP. Granaticin, an antibiotic 
      which prevents the charging of leucyl-transfer ribonucleic acid, was used to show 
      that the stringent response inhibited the formation of xanthosine monophosphate 
      from inosine monophosphate. It prevented the accumulation of xanthosine 
      monophosphate in decoyinine-treated cultures of the stringent strain but not in 
      those of the relaxed strain.
FAU - Lopez, J M
AU  - Lopez JM
FAU - Dromerick, A
AU  - Dromerick A
FAU - Freese, E
AU  - Freese E
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Bacteriol
JT  - Journal of bacteriology
JID - 2985120R
RN  - 0 (Culture Media)
RN  - 0 (Glutamates)
RN  - 0 (Guanine Nucleotides)
RN  - 0 (Lactates)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 1.1.1.205 (IMP Dehydrogenase)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Bacillus subtilis/*physiology
MH  - Culture Media
MH  - Glutamates/metabolism
MH  - Guanine Nucleotides/metabolism
MH  - Guanosine Triphosphate/*metabolism
MH  - IMP Dehydrogenase/metabolism
MH  - Lactates/metabolism
MH  - Spores, Bacterial/physiology
PMC - PMC217003
EDAT- 1981/05/01 00:00
MHDA- 1981/05/01 00:01
PMCR- 1981/05/01
CRDT- 1981/05/01 00:00
PHST- 1981/05/01 00:00 [pubmed]
PHST- 1981/05/01 00:01 [medline]
PHST- 1981/05/01 00:00 [entrez]
PHST- 1981/05/01 00:00 [pmc-release]
AID - 10.1128/jb.146.2.605-613.1981 [doi]
PST - ppublish
SO  - J Bacteriol. 1981 May;146(2):605-13. doi: 10.1128/jb.146.2.605-613.1981.