PMID- 6117075
OWN - NLM
STAT- MEDLINE
DCOM- 19811221
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 78
IP  - 7
DP  - 1981 Jul
TI  - Selection of rat hepatoma cells defective in hormone-regulated production of 
      mouse mammary tumor virus RNA.
PG  - 4349-53
AB  - We have been studying the mechanism of glucocorticoid hormone action by using 
      mouse mammary tumor virus (MMTV)-infected rat hepatoma cells as a model system. 
      J2.17, a clonal cell line that contains one MMTV provirus, induces tyrosine 
      aminotransferase (TyrATase; L-tyrosine:2-oxoglutarate aminotransferase, EC 
      2.6.1.5), viral RNA, and the cell surface viral glycoprotein gp52 in response to 
      dexamethasone. Using a fluorescence-activated cell sorter and a rabbit antiserum 
      directed against gp52, we selected a cell population that displays a reduced 
      hormone-mediated increase in cell surface gp52. Fourteen clones of this 
      population were assayed for induction of viral gp52 and RNA and of cellular 
      TyrATase. The results of these assays revealed that the clones display a variety 
      of responses to hormone. One clone has retained wild-type responses of both 
      TyrATase and gp52. Six clones exhibit coordinately reduced or abolished responses 
      of both markers. Seven clones show normal induction of TyrATase but reduced or 
      undetectable induction of gp52. These latter clones exhibit reduced production of 
      MMTV RNA and thus may represent a unique class of variants defective in the 
      regulation of MMTV gene expression.
FAU - Grove, J R
AU  - Grove JR
FAU - Ringold, G M
AU  - Ringold GM
LA  - eng
GR  - GM 07149/GM/NIGMS NIH HHS/United States
GR  - GM 25821/GM/NIGMS NIH HHS/United States
GR  - RR 5353/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Glycoproteins)
RN  - 0 (Viral Proteins)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 2.6.1.5 (Tyrosine Transaminase)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Dexamethasone/*pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Genes, Viral
MH  - Glycoproteins/genetics
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Mutation
MH  - Neoplasms, Experimental/genetics
MH  - Neoplasms, Hormone-Dependent/*genetics
MH  - Rats
MH  - Transcription, Genetic/drug effects
MH  - Tyrosine Transaminase/*genetics
MH  - Viral Proteins/*genetics
MH  - Virus Replication
PMC - PMC319787
EDAT- 1981/07/01 00:00
MHDA- 1981/07/01 00:01
PMCR- 1982/01/01
CRDT- 1981/07/01 00:00
PHST- 1981/07/01 00:00 [pubmed]
PHST- 1981/07/01 00:01 [medline]
PHST- 1981/07/01 00:00 [entrez]
PHST- 1982/01/01 00:00 [pmc-release]
AID - 10.1073/pnas.78.7.4349 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1981 Jul;78(7):4349-53. doi: 10.1073/pnas.78.7.4349.