PMID- 6132543
OWN - NLM
STAT- MEDLINE
DCOM- 19830505
LR  - 20041117
IS  - 0001-723X (Print)
IS  - 0001-723X (Linking)
VI  - 26
IP  - 6
DP  - 1982 Dec
TI  - Restricted replication of the E5"14" clone of Langat TP21 virus (from the 
      tick-borne encephalitis complex) in CNS of subadult mice.
PG  - 488-96
AB  - Subadult ICR mice were infected with the low virulent Langat virus TP21 E5 strain 
      clone "14" belonging to the tick-borne encephalitis (TBE) complex by subcutaneous 
      (s.c.) and intracerebral (i.c.) routes. From 5 to 6 days post infection (p.i.), 
      no virus was detected in cultured brain fragments of mice, which received 10(6) 
      ic LD50 into interscapular area. Acute lethal encephalitis with lesions confined 
      to the vicinity of the inoculation area (parietal cortex, basal ganglia, 
      thalamus) has developed in all mice, which received greater than or equal to 3 
      PFU of the virus by i.c. route. However, no virus was recovered from the cultured 
      fragments of brain stem and cerebellum of these animals, although direct 
      isolation attempts were regularly positive from brain cortex and basal ganglia. 
      Survivors, which did not succumb to i.c. administration of approximately equal to 
      1 ic LD50 (0.3 PFU) of the attenuated Langat strain were autopsied between 53-74 
      days p.i. Attempts to isolate the virus from cultured fragments of brain cortex 
      and basal ganglia remained negative despite of the presence of focal residual 
      histological lesions in g. hippocampi in 15% of of animals examined.
FAU - Rajcani, J
AU  - Rajcani J
FAU - Mayer, V
AU  - Mayer V
FAU - Weismanova, E
AU  - Weismanova E
LA  - eng
PT  - Journal Article
PL  - Slovakia
TA  - Acta Virol
JT  - Acta virologica
JID - 0370401
SB  - IM
MH  - Animals
MH  - Brain/*microbiology/pathology
MH  - Encephalitis/etiology
MH  - Encephalitis Viruses, Tick-Borne/isolation & 
      purification/pathogenicity/*physiology
MH  - Flavivirus/*physiology
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Organ Culture Techniques
MH  - Togaviridae Infections/*microbiology
MH  - Virus Replication
EDAT- 1982/12/01 00:00
MHDA- 1982/12/01 00:01
CRDT- 1982/12/01 00:00
PHST- 1982/12/01 00:00 [pubmed]
PHST- 1982/12/01 00:01 [medline]
PHST- 1982/12/01 00:00 [entrez]
PST - ppublish
SO  - Acta Virol. 1982 Dec;26(6):488-96.