PMID- 6133450
OWN - NLM
STAT- MEDLINE
DCOM- 19830610
LR  - 20181130
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 244
IP  - 5
DP  - 1983 May
TI  - Beta-adrenergic modulation of mucin secretion in cat trachea.
PG  - C391-8
AB  - The mechanism of beta-adrenergic regulation of mucin secretion was investigated 
      in cat trachea in vitro. beta-Adrenergic agonists increased the release of 
      [35S]SO4-radiolabeled mucin and mucosa-submucosal adenosine 3',5'-cyclic 
      monophosphate (cAMP) levels in a dose- and time-dependent manner. The relative 
      potencies and efficacies of l-isoproterenol, l-epinephrine, l-norepinephrine, 
      terbutaline, and dobutamine for physiological and biochemical events were 
      similar. The effect of these agonists were blocked by d-l-propranolol. 
      3-Isobutyl-l-methylxanthine (IBMX) and 8-bromo-cAMP mimicked the effects of the 
      agonists on mucin release. IBMX increased cAMP levels and potentiated the 
      increase in cAMP levels effected by beta-adrenergic agonists. The half-maximal 
      effects of l-isoproterenol on cAMP levels and mucin release were attained at 1.6 
      and 8.8 min, respectively. Three major mucosa-submucosal proteins of apparent 
      molecular weights of 49,000, 54,000, and 59,000 daltons displayed reduced binding 
      of the photoaffinity label 8-N3-[32P]cAMP when endogenous cAMP levels were 
      increased with l-isoproterenol and/or IBMX. The first two proteins correspond in 
      electrophoretic mobility to the regulatory subunits of type I and type II 
      cAMP-dependent protein kinases, respectively. The 59,000-dalton cAMP binding 
      protein may be the phosphorylated form of the regulatory subunit of type II 
      cAMP-dependent protein kinase. These data are consistent with the hypothesis that 
      beta-adrenergic modulation of tracheal mucin release is mediated by cAMP and 
      suggest that activation of cAMP-dependent protein kinases may be involved in the 
      neurohormonal effects.
FAU - Liedtke, C M
AU  - Liedtke CM
FAU - Rudolph, S A
AU  - Rudolph SA
FAU - Boat, T F
AU  - Boat TF
LA  - eng
GR  - HL-07415/HL/NHLBI NIH HHS/United States
GR  - HL-21995/HL/NHLBI NIH HHS/United States
GR  - HL-26759/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Adrenergic beta-Agonists)
RN  - 0 (Adrenergic beta-Antagonists)
RN  - 0 (Mucins)
RN  - 0 (Sulfates)
RN  - E0399OZS9N (Cyclic AMP)
RN  - L628TT009W (Isoproterenol)
RN  - X4W3ENH1CV (Norepinephrine)
RN  - YKH834O4BH (Epinephrine)
SB  - IM
MH  - Adrenergic beta-Agonists/*pharmacology
MH  - Adrenergic beta-Antagonists/*pharmacology
MH  - Animals
MH  - Cats
MH  - Cyclic AMP/metabolism
MH  - Epinephrine/pharmacology
MH  - Female
MH  - Isoproterenol/pharmacology
MH  - Kinetics
MH  - Mucins/*metabolism
MH  - Mucous Membrane/drug effects/metabolism
MH  - Norepinephrine/pharmacology
MH  - Sulfates/metabolism
MH  - Trachea/*metabolism
EDAT- 1983/05/01 00:00
MHDA- 1983/05/01 00:01
CRDT- 1983/05/01 00:00
PHST- 1983/05/01 00:00 [pubmed]
PHST- 1983/05/01 00:01 [medline]
PHST- 1983/05/01 00:00 [entrez]
AID - 10.1152/ajpcell.1983.244.5.C391 [doi]
PST - ppublish
SO  - Am J Physiol. 1983 May;244(5):C391-8. doi: 10.1152/ajpcell.1983.244.5.C391.